p16INK4a和p14ARF在皮肤基底细胞癌和鳞状细胞癌中的表达及其临床意义
发布时间：2019-04-01 14:19
【摘要】：目的:基因突变在肿瘤的发病机制中起着重要作用,本文旨在探讨pl6INK4a和p14ARF在皮肤基底细胞癌(BCC)和鳞状细胞癌(SCC)中的表达特征及其临床意义。 方法:采用免疫组织化学S-P法对25例皮肤基底细胞癌(BCC)、18例皮肤鳞状细胞癌(SCC)标本和20例正常皮肤标本进行pl6INK4a和p14ARF蛋白的检测,通过光学显微镜观察免疫组织化学表达情况并应用统计学方法对结果进行分析。 结果:pl6INK4a和p14ARF蛋白染色阳性产物都定位于细胞核内。①pl6INK4a蛋白在BCC和SCC组的阳性表达率显著低于正常皮肤组,差异有统计学意义(P0.05)。②p14ARF蛋白在BCC和SCC组的阳性表达率显著低于正常皮肤组,差异有统计学意义( p0.05)。③pl6INK4a和p14ARF蛋白阳性表达率在BCC和SCC组及正常组织中无明显相关性(p0.05)。 结论: pl6INK4a和p14ARF在皮肤BCC和SCC中的低表达,提示pl6INK4a和p14ARF基因突变在皮肤BCC和SCC的发生、发展中起着重要作用,有可能成为一种良好的皮肤肿瘤标志物。
[Abstract]:Aim: gene mutations play an important role in the pathogenesis of tumors. The aim of this study was to investigate the expression characteristics and clinical significance of pl6INK4a and p14ARF in (BCC) and (SCC) in cutaneous basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Methods: immunohistochemical method was used to detect pl6INK4a and p14ARF protein in 25 cases of basal cell carcinoma (BCC), 18 cases of squamous cell carcinoma of skin (SCC) and 20 cases of normal skin. The immunohistochemical expression was observed by optical microscope and the results were analyzed by statistical method. Results: the positive staining products of pl6INK4a and p14ARF protein were located in nucleus. 1the positive expression rate of pl6INK4a protein in BCC and SCC group was significantly lower than that in normal skin group. The positive expression rate of p14ARF protein in BCC and SCC group was significantly lower than that in normal skin group (P0.05). There was no significant correlation between the positive expression of pl6INK4a and p14ARF protein in BCC and SCC group and normal tissues (p0.05). Conclusion: the low expression of pl6INK4a and p14ARF in skin BCC and SCC suggests that pl6INK4a and p14ARF gene mutations play an important role in the occurrence and development of BCC and SCC and may be a good marker of skin tumor.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.5
，
本文编号：2451630
[Abstract]:Aim: gene mutations play an important role in the pathogenesis of tumors. The aim of this study was to investigate the expression characteristics and clinical significance of pl6INK4a and p14ARF in (BCC) and (SCC) in cutaneous basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Methods: immunohistochemical method was used to detect pl6INK4a and p14ARF protein in 25 cases of basal cell carcinoma (BCC), 18 cases of squamous cell carcinoma of skin (SCC) and 20 cases of normal skin. The immunohistochemical expression was observed by optical microscope and the results were analyzed by statistical method. Results: the positive staining products of pl6INK4a and p14ARF protein were located in nucleus. 1the positive expression rate of pl6INK4a protein in BCC and SCC group was significantly lower than that in normal skin group. The positive expression rate of p14ARF protein in BCC and SCC group was significantly lower than that in normal skin group (P0.05). There was no significant correlation between the positive expression of pl6INK4a and p14ARF protein in BCC and SCC group and normal tissues (p0.05). Conclusion: the low expression of pl6INK4a and p14ARF in skin BCC and SCC suggests that pl6INK4a and p14ARF gene mutations play an important role in the occurrence and development of BCC and SCC and may be a good marker of skin tumor.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.5
，
本文编号：2451630
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