慢性特发性荨麻疹患者血清IL-6、IL-12P70、β-内啡肽及组胺测定及意义
发布时间：2019-04-19 04:49
【摘要】： 目的通过测定慢性特发性荨麻疹(CIU)患者血清中IL-6、IL-12P70、β-内啡肽及组胺的水平,初步探讨神经精神因素在CIU发病机制中的作用。 方法选择门诊确诊的CIU患者共62例(其中在取血时处于发作状态者35例,非发作状态者27例),选择健康志愿者34例作为对照组。应用ELISA法检测发作状态、非发作状态CIU患者及健康对照组血清IL-6、IL-12P70、β-内啡肽及组胺的含量。应用SPSS17.0统计软件包进行统计学处理。 结果①IL-6水平：CIU发作状态组、非发作状态组、健康对照组血清水平分别为458.19±72.79pg/ml,408.19±68.79pg/ml,432.32±92.38pg/ml,差异无统计学意义(p＞0.05)。②IL-12P70水平：CIU发作状态组、非发作状态组、健康对照组血清IL-12P70水平分别为286.39±138.06pg/ml,328.39±112.96pg/ml,366.76±115.82pg/ml,差异有统计学意义(p0.05),其中发作状态组血清IL-12P70水平低于健康对照组,差异有统计学意义(p0.05)。非发作状态组与健康对照组血清IL-12P70水平差异无统计学意义(p0.05)。非发作状态组与发作状态组血清IL-12P70水平差异无统计学意义(p0.05)。③β-内啡肽水平：CIU发作状态组、非发作状态组、健康对照组血清β-内啡肽水平分别19.42±5.93ng/ml,18.70±6.12ng/ml,15.70±4.92ng/ml,差异有统计学意义(p0.05),其中发作状态组及非发作状态组血清β-内啡肽水平均高于健康对照组,差异有统计学意义(p0.05)。发作状态组与非发作状态组血清β-内啡肽水平差异无统计学意义(p0.05)。④组胺水平：CIU发作状态组、非发作状态组、健康对照组血清组胺水平分别为34.03±14.32ng/ml,27.70±9.32ng/ml,20.61±10.71ng/ml,差异有统计学意义(p0.05),其中发作状态组血清组胺水平高于健康对照组,差异有统计学意义(p0.01)。非发作状态组血清组胺水平高于健康对照组,差异有统计学意义(p0.05)。发作状态组血清组胺水平高于非发作状态组,差异有统计学意义(p＜0.05)。⑤相关性分析：发作状态组血清β-内啡肽与组胺水平成正相关(r=0.712,p0.01),组胺与IL-12P70水平成负相关(r=-0.357,p0.05),β-内啡肽与IL-12P70水平无相关性(r=0.167,p0.05)。非发作状态组血清β-内啡肽与组胺水平成正相关(r=0.615,p＜0.05)。非发作状态组血清组胺与IL-12P70无明显相关性(r=0.128,p0.05)。非发作状态组血清β-内啡肽与IL-12P70水平无相关性(r=0.147,p＞0.05)。 结论①血清IL-12P70水平降低,提示Th1/Th2免疫失衡可能在CIU的发病机制中起重要作用。②组胺在CIU的发病中起重要作用。③组胺可能通过抑制细胞免疫,造成机体T1/T2免疫失衡,从而诱发和(或)加重CIU的发生。④血清β-内啡肽水平升高初步提示神经精神因素在CIU的发病中的作用。⑤神经精神因素可能通过引起机体Th1/Th2免疫失衡和(或)直接刺激肥大细胞脱颗粒释放组胺来诱发和(或)加重CIU患者的病情。
[Abstract]:Aim to investigate the role of neuropsychological factors in the pathogenesis of chronic idiopathic urticaria (CIU) by measuring the serum levels of IL-6,IL-12P70, 尾-endorphin and histamine in patients with chronic idiopathic urticaria. Methods A total of 62 CIU patients (35 in seizure state and 27 in non-seizure state) were enrolled in this study. 34 healthy volunteers were selected as control group. The levels of serum IL-6,IL-12P70, 尾-endorphin and histamine in patients with and without CIU and healthy controls were detected by ELISA method. SPSS17.0 statistical software package was used for statistical processing. Results the serum levels of 1IL-6 were 458.19 卤72.79 PG / ml, 408.19 卤68.79 PG / ml, 432.32 卤92.38 PG / ml in CIU attack group, non-attack state group and healthy control group, respectively. The levels of serum 2IL-12P70 were 286.39 卤138.06 PG / ml, 328.39 卤112.96 PG / ml, 366.76 卤115.82 PG / ml in CIU attack group, non-attack state group and healthy control group, respectively (p > 0.05). The difference was statistically significant (p0.05), in which the level of serum IL-12P70 in the attack state group was lower than that in the healthy control group, the difference was statistically significant (p0.05). There was no significant difference in serum IL-12P70 level between non-seizure group and healthy control group (p0.05). There was no significant difference in serum IL-12P70 level between the non-seizure group and the attack state group (p0.05). 3 尾-endorphin levels: CIU attack state group, non-seizure state group, 3 尾-endorphin level. The serum 尾-endorphin levels in healthy control group were 19.42 卤5.93 ng / ml, 18.70 卤6.12 ng / ml, 15.70 卤4.92 ng / ml, respectively (p0.05). The serum 尾-endorphin levels in the seizure group and the non-seizure group were significantly higher than those in the healthy control group (p0.05). There was no significant difference in serum 尾-endorphin levels between seizure group and non-seizure group (p0.05). 4 histamine levels were 34.03 卤14.32 ng / ml in CIU attack group, 34.03 卤14.32 ng / ml in non-seizure state group, and 34.03 卤14.32 ng / ml in healthy control group. 27.70 卤9.32ng / ml and 20.61 卤10.71ng / ml, the difference was statistically significant (p0.05), in which the serum histamine level in the attack group was higher than that in the healthy control group (p0.01). The serum histamine level in non-seizure group was significantly higher than that in healthy control group (p0.05). The serum histamine level in the seizure group was significantly higher than that in the non-seizure group (p < 0.05). 5. There was a positive correlation between the serum 尾-endorphin level and histamine level in the seizure group (r = 0.712, p0.01), and there was a positive correlation between 尾-endorphin and histamine level in the seizure group (p < 0.05). Histamine was negatively correlated with the level of IL-12P70 (r = 0.357, p0.05), but 尾-endorphin was not correlated with the level of IL-12P70 (r = 0.167, p0.05). There was a positive correlation between serum 尾-endorphin and histamine level in non-seizure group (r = 0.615, p < 0.05). There was no significant correlation between serum histamine and IL-12P70 in non-seizure group (r = 0.128, p 0.05). There was no correlation between serum 尾-endorphin and IL-12P70 (r = 0.147, p > 0.05). Conclusion 1 the decrease of serum IL-12P70 level suggests that Th1/Th2 immune imbalance may play an important role in the pathogenesis of CIU. 2 histamine may play an important role in the pathogenesis of CIU. 3 histamine may inhibit cellular immunity. Causing immune imbalance in the body's T1/T2, 4 the increase of serum 尾-endorphin level suggests the role of neuropsychological factors in the pathogenesis of CIU. 5 the neuropsychological factors may be related to the immune imbalance of Th1/Th2 and the pathogenesis of CIU. 5. The increase of serum 尾-endorphin levels suggests that neuropsychological factors may play an important role in the pathogenesis of CIU. 5. (or) stimulating mast cells to degranulate and release histamine directly to induce and / or aggravate the condition of patients with CIU.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.24
本文编号：2460616
[Abstract]:Aim to investigate the role of neuropsychological factors in the pathogenesis of chronic idiopathic urticaria (CIU) by measuring the serum levels of IL-6,IL-12P70, 尾-endorphin and histamine in patients with chronic idiopathic urticaria. Methods A total of 62 CIU patients (35 in seizure state and 27 in non-seizure state) were enrolled in this study. 34 healthy volunteers were selected as control group. The levels of serum IL-6,IL-12P70, 尾-endorphin and histamine in patients with and without CIU and healthy controls were detected by ELISA method. SPSS17.0 statistical software package was used for statistical processing. Results the serum levels of 1IL-6 were 458.19 卤72.79 PG / ml, 408.19 卤68.79 PG / ml, 432.32 卤92.38 PG / ml in CIU attack group, non-attack state group and healthy control group, respectively. The levels of serum 2IL-12P70 were 286.39 卤138.06 PG / ml, 328.39 卤112.96 PG / ml, 366.76 卤115.82 PG / ml in CIU attack group, non-attack state group and healthy control group, respectively (p > 0.05). The difference was statistically significant (p0.05), in which the level of serum IL-12P70 in the attack state group was lower than that in the healthy control group, the difference was statistically significant (p0.05). There was no significant difference in serum IL-12P70 level between non-seizure group and healthy control group (p0.05). There was no significant difference in serum IL-12P70 level between the non-seizure group and the attack state group (p0.05). 3 尾-endorphin levels: CIU attack state group, non-seizure state group, 3 尾-endorphin level. The serum 尾-endorphin levels in healthy control group were 19.42 卤5.93 ng / ml, 18.70 卤6.12 ng / ml, 15.70 卤4.92 ng / ml, respectively (p0.05). The serum 尾-endorphin levels in the seizure group and the non-seizure group were significantly higher than those in the healthy control group (p0.05). There was no significant difference in serum 尾-endorphin levels between seizure group and non-seizure group (p0.05). 4 histamine levels were 34.03 卤14.32 ng / ml in CIU attack group, 34.03 卤14.32 ng / ml in non-seizure state group, and 34.03 卤14.32 ng / ml in healthy control group. 27.70 卤9.32ng / ml and 20.61 卤10.71ng / ml, the difference was statistically significant (p0.05), in which the serum histamine level in the attack group was higher than that in the healthy control group (p0.01). The serum histamine level in non-seizure group was significantly higher than that in healthy control group (p0.05). The serum histamine level in the seizure group was significantly higher than that in the non-seizure group (p < 0.05). 5. There was a positive correlation between the serum 尾-endorphin level and histamine level in the seizure group (r = 0.712, p0.01), and there was a positive correlation between 尾-endorphin and histamine level in the seizure group (p < 0.05). Histamine was negatively correlated with the level of IL-12P70 (r = 0.357, p0.05), but 尾-endorphin was not correlated with the level of IL-12P70 (r = 0.167, p0.05). There was a positive correlation between serum 尾-endorphin and histamine level in non-seizure group (r = 0.615, p < 0.05). There was no significant correlation between serum histamine and IL-12P70 in non-seizure group (r = 0.128, p 0.05). There was no correlation between serum 尾-endorphin and IL-12P70 (r = 0.147, p > 0.05). Conclusion 1 the decrease of serum IL-12P70 level suggests that Th1/Th2 immune imbalance may play an important role in the pathogenesis of CIU. 2 histamine may play an important role in the pathogenesis of CIU. 3 histamine may inhibit cellular immunity. Causing immune imbalance in the body's T1/T2, 4 the increase of serum 尾-endorphin level suggests the role of neuropsychological factors in the pathogenesis of CIU. 5 the neuropsychological factors may be related to the immune imbalance of Th1/Th2 and the pathogenesis of CIU. 5. The increase of serum 尾-endorphin levels suggests that neuropsychological factors may play an important role in the pathogenesis of CIU. 5. (or) stimulating mast cells to degranulate and release histamine directly to induce and / or aggravate the condition of patients with CIU.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.24
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