不同底物体外诱导不同基因型红色毛癣菌产生角蛋白酶的活性比较

发布时间：2019-04-19 17:19

【摘要】：目的:通过探讨红色毛癣菌体外分泌角蛋白酶的活性与诱导底物及其基因型的关系,从而了解红色毛癣菌的侵袭力与感染部位及其基因型的关系。方法:分别以指甲、趾甲、皮屑和头发为底物,对不同基因型红色毛癣菌T1a和T1b进行体外培养,利用keratin-azure和酶标仪检测其产生的角蛋白酶活性。结果:不同底物诱导红色毛癣菌T1a和T1b产生角蛋白酶活性的高低比较,指(趾)甲高于皮屑(P0.05),皮屑高于头发(P0.05),指甲和趾甲之间差异无统计学意义(P0.05)。同一底物诱导菌株T1a和T1b产生的角蛋白酶活性间差异无统计学意义(P0.05)。结论:不同底物诱导的角蛋白酶活性不同;红色毛癣菌的侵袭力可能与其感染部位所含的诱导底物有关,而与基因型无关。
[Abstract]:Aim: to investigate the relationship between the activity of exocrine keratinase and the substrate and genotype of Trichophyton rubrum, so as to find out the relationship between invasive ability of Trichophyton rubrum and infection site and genotype of Trichophyton rubrum. Methods: different genotypes of Trichophyton rubrum T1a and T1b were cultured in vitro using fingernails, toenails, dandruff and hair as substrates. The activity of keratinase produced by T1a and T1b were detected by keratin-azure and enzyme labeling apparatus. Results: the activity of keratinase produced by Trichophyton rubrum T1a and T1b induced by different substrates was higher than that of dandruff (P0.05) and dandruff (P0.05), and that of trichophyton rubrum was higher than that of hair (P0.05). There was no significant difference between nail and toenail (P0.05). There was no significant difference in keratinase activity between T1a and T1b induced by the same substrate (P0.05). Conclusion: the keratinase activity induced by different substrates is different, and the invasiveness of Trichophyton rubrum may be related to the substrate contained in the infected site, but not to the genotype of Trichophyton rubrum.
【作者单位】：濮阳市油田总医院皮肤科;中山大学附属第三医院皮肤科;
【分类号】：R756.9

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