窄谱UVB照射对寻常型银屑病患者MCP-4及T细胞CCR-6表达的影响
发布时间：2019-05-17 08:30
【摘要】： 目的:银屑病是一种由多基因遗传决定的、多环境因素刺激诱导的免疫异常性慢性炎症性增生性皮肤病,其病理过程十分复杂,涉及诸多细胞因子及免疫分子形成的网络,趋化因子及其受体在其中是至关重要的一环。本文旨在观察UVB窄波治疗仪治疗前后患者血清单核细胞趋化蛋白4(MCP-4)及外周血T细胞上趋化因子受体6(CCR6)表达率水平的影响,并分别与正常人群对比,探讨它们在银屑病发病机制中可能发挥的作用。 材料和方法:选取寻常型银屑病患者及正常人群各24例,分别于治疗前采集血样标本,以夹心酶联免疫吸附法测定其血清中MCP-4的含量,以流式细胞仪测定其外周血T细胞上中CCR6的表达率。 NB-UVB治疗:采用UV100L(TL01)(德国沃曼公司制造,波长310-315nm,峰值为311nm)进行NB-UVB照射。首次剂量取最小有效量0.3焦耳/cm2,以后每次增加0.1焦耳/cm2,最终剂量不超过3焦耳/cm2,每周照射3次,总疗程平均36次。照射过程中嘱患者戴上遮光眼睛,避免伤及眼睛。治疗过程中,出现红斑+(3-7天,淡粉或淡棕色,无压痛无水肿)为最理想反应,出现红斑++(明确红斑,无水肿无压痛)应停止治疗,临床可按日光性灼伤处理。 经过12周NB-UVB治疗后,再次采集银屑病患者血样标本检测其血清中MCP-4的含量及外周血T细胞上CCR6的表达率,并以银屑病严重程度PASI评分标准评分,分析寻常型银屑病患者在治疗前后及与正常人群血清MCP-4的含量及外周血T细胞CCR6的表达率对比,以SPSS17.0软件包分析统计学结果。 结果:24例寻常型银屑病患者患者血清MCP-4水平为255.35±79.31 pg/ml,正常人对照组血清MCP-4水平为32.43±10.41pg/ml,两组差异有统计学意义(t= 13.145,P㩳0.05)。经NB-UVB治疗12周后,银屑病患者MCP-4水平明显下降88.19±23.97 pg/ml,差异有统计学意义(t=9.229,P 0.05)。24例寻常型银屑病患者患者外周血T细胞CCR-6表达率为(42.10±11.08)%,正常人对照组外周血T细胞CCR-6表达率为(11.03±1.01)%,两组差异有统计学意义(t=13.02,P㩳0.05)。经NB-UVB治疗12周后,银屑病患者外周血T细胞CCR-6表达率无明显下降,为(40.05±4.52)%差异有无统计学意义(t= 1.43,P㧐0.05)。 结论:趋化因子通过其相应受体对多种细胞如淋巴细胞、单核细胞、中性粒细胞、树突状细胞等的趋化作用而在多种疾病的免疫反应及炎症损伤中发挥着重要作用。银屑病是一种慢性炎症性自身免疫性疾病,涉及诸多细胞因子及免疫分子。本实验提示,寻常型银屑病患者外周血中MCP-4较正常人群高表达,且随病情的变化而变化,可能与寻常型银屑病的发病机制有着密切关系,促使了银屑病的发生发展,可以作为银屑病病情变化的参考指标。另外,寻常型银屑病患者外周血PBMC中T细胞上CCR6的表达率明显高于正常人群,可能与银屑病的发病机制有着密切关系,但其不随病情的变化而变化,故不能作为银屑病病情变化的参考指标。
[Abstract]:Objective: psoriasis is a kind of chronic inflammatory hyperplastic dermatosis induced by polygenetic and multi-environmental stimuli. The pathological process of psoriasis is very complex, involving a network of cytokines and immune molecules. Chemokines and their receptors are a vital part of them. The purpose of this study was to observe the effect of UVB narrow wave therapeutic apparatus on the expression of serum monocyte chemoattractant protein 4 (MCP-4) and chemokine receptor 6 (CCR6) on peripheral blood T cells before and after treatment, and to compare it with that of normal population. To explore the possible role of them in the pathogenesis of psoriasis. Materials and methods: blood samples were collected from 24 patients with psoriasis vulgaris and 24 normal people before treatment, and the content of MCP-4 in serum was determined by sandwich enzyme-linked immunosorbent assay (Elisa). The expression rate of CCR6 in peripheral blood T cells was measured by flow cytometry. NB-UVB treatment: NB-UVB was irradiated with UV100L (TL01) (made by Warman, Germany, wavelength 310-315 nm, peak 311nm). After the minimum effective dose of 0.3joule / cm2, was taken for the first time, the final dose of 0.1joule / cm2, was not more than 3 joules / cm2, three times a week, and the total course of treatment was 36 times on average. In the process of irradiation, patients are told to wear shaded eyes to avoid injury to the eyes. In the course of treatment, erythema (3 鈮，
本文编号：2478951
[Abstract]:Objective: psoriasis is a kind of chronic inflammatory hyperplastic dermatosis induced by polygenetic and multi-environmental stimuli. The pathological process of psoriasis is very complex, involving a network of cytokines and immune molecules. Chemokines and their receptors are a vital part of them. The purpose of this study was to observe the effect of UVB narrow wave therapeutic apparatus on the expression of serum monocyte chemoattractant protein 4 (MCP-4) and chemokine receptor 6 (CCR6) on peripheral blood T cells before and after treatment, and to compare it with that of normal population. To explore the possible role of them in the pathogenesis of psoriasis. Materials and methods: blood samples were collected from 24 patients with psoriasis vulgaris and 24 normal people before treatment, and the content of MCP-4 in serum was determined by sandwich enzyme-linked immunosorbent assay (Elisa). The expression rate of CCR6 in peripheral blood T cells was measured by flow cytometry. NB-UVB treatment: NB-UVB was irradiated with UV100L (TL01) (made by Warman, Germany, wavelength 310-315 nm, peak 311nm). After the minimum effective dose of 0.3joule / cm2, was taken for the first time, the final dose of 0.1joule / cm2, was not more than 3 joules / cm2, three times a week, and the total course of treatment was 36 times on average. In the process of irradiation, patients are told to wear shaded eyes to avoid injury to the eyes. In the course of treatment, erythema (3 鈮，
本文编号：2478951
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