1320nmND：YAG激光治疗皮肤光老化的动物实验研究
发布时间：2019-06-04 14:22
【摘要】：背景与目的 皮肤光老化主要指人的皮肤受紫外线等外界环境因素的影响出现衰老的现象,主要表现为皮肤粗糙、皱纹色斑出现等,不仅影响一个人的外表,而且与皮肤癌有正相关性,它的发病机制目前不完全清楚。皮肤光老化的治疗方法较多,YAG激光治疗是近几年发展起来的除皱紧肤术,具有创伤小,临床愈合快的特点,在临床上治疗皮肤光老化取得了不错的疗效,但其具体治疗机制目前并不清楚。本研究通过大鼠皮肤光老化动物模型,进一步研究皮肤光老化和YAG激光治疗的机制,以期对皮肤光老化的临床治疗有所帮助。 方法 选取雄性SD大鼠40只作为研究对象,随机选取10只作为对照组,其余30只复制皮肤光老化模型,模型复制成功后再随机分成三组(光老化组、激光治疗组、自然恢复组),每只大鼠皮肤处理部位分别做组织病理HE染色,并提取RNA,逆转录成cDNA,做SYBR Green Ⅰ real time PCR检测四组大鼠皮肤中β-actin、Ⅰ型胶原、Ⅲ型胶原、CTGF、TGF-β1的mRNA表达情况。 结果 肉眼观察：光老化组大鼠背部皮肤增厚,粗糙,出现较深皱纹,经YAG激光治疗后大鼠皮肤光老化症状减轻,而自然恢复组变化不明显；显微镜观察：光老化组大鼠皮肤HE染色,表皮紊乱,疏松增厚、厚薄不均,真皮胶原纤维断裂、排列扭曲紊乱,有炎性细胞浸润,YAG激光治疗后,症状明显改善；与对照组相比,光老化组Ⅰ型胶原、Ⅲ型胶原、CTGF、TGF-β1mRNA表达明显降低,YAG激光治疗后,这些基因mRNA表达明显升高,激光治疗组与自然恢复组相比,除CTGF外,Ⅰ型胶原、Ⅲ型胶原、TGF-p的mRNA表达均明显升高。 结论 1320nmND:YAG激光治疗大鼠皮肤光老化效果显著,其治疗机制可能与其能够刺激皮肤中Ⅰ型胶原、Ⅲ型胶原、TGF-β1mRNA表达升高有关。
[Abstract]:Background and objective skin photoaging mainly refers to the aging phenomenon of human skin affected by ultraviolet and other external environmental factors, mainly manifested in rough skin, wrinkle spots and so on, which not only affects a person's appearance. And there is a positive correlation with skin cancer, its pathogenesis is not fully clear. There are many treatments for skin photoaging. YAG laser therapy is a wrinkle tightening technique developed in recent years, which has the characteristics of small trauma and rapid clinical healing, and has achieved good results in clinical treatment of skin photoaging. However, the specific treatment mechanism is not clear at present. In this study, the mechanism of skin photoaging and YAG laser therapy was further studied through the animal model of skin photoaging in rats, in order to be helpful to the clinical treatment of skin photoaging. Methods 40 male SD rats were randomly selected as control group and the other 30 rats as skin photoaging model. After successful replication of the model, they were randomly divided into three groups: photoaging group and laser treatment group. In the natural recovery group, pathological HE staining was performed in the skin treatment sites of each rat, and cDNA, was extracted from the skin of each rat. SYBR Green 鈪，
本文编号：2492781
[Abstract]:Background and objective skin photoaging mainly refers to the aging phenomenon of human skin affected by ultraviolet and other external environmental factors, mainly manifested in rough skin, wrinkle spots and so on, which not only affects a person's appearance. And there is a positive correlation with skin cancer, its pathogenesis is not fully clear. There are many treatments for skin photoaging. YAG laser therapy is a wrinkle tightening technique developed in recent years, which has the characteristics of small trauma and rapid clinical healing, and has achieved good results in clinical treatment of skin photoaging. However, the specific treatment mechanism is not clear at present. In this study, the mechanism of skin photoaging and YAG laser therapy was further studied through the animal model of skin photoaging in rats, in order to be helpful to the clinical treatment of skin photoaging. Methods 40 male SD rats were randomly selected as control group and the other 30 rats as skin photoaging model. After successful replication of the model, they were randomly divided into three groups: photoaging group and laser treatment group. In the natural recovery group, pathological HE staining was performed in the skin treatment sites of each rat, and cDNA, was extracted from the skin of each rat. SYBR Green 鈪，
本文编号：2492781
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