Psoriasin在扁平苔藓、急性发热性嗜中性皮病中的表达和意义

发布时间：2019-06-05 12:09

【摘要】：Psoriasin为S100蛋白家族成员，于1991年在银屑病患者皮损中首次发现。20年来随着不断研究，psoriasin在特应性皮炎、蕈样肉芽肿等多种炎症鳞屑性皮肤病及皮肤癌症等疾病中被发现，其不仅与角质细胞增殖分化有关，并且具有趋化中性粒细胞和CD4+T细胞至角质层的生物学功能，近年来还发现psoriasin参与了皮肤的抗菌、调节免疫等作用。Psoriasin与皮肤病的相关性越来越受到重视，但是其在炎症性皮肤病中的具体作用机制尚不明确，对于真皮层炎症的作用尚有待进一步研究。目的：探讨psoriasin在扁平苔藓、急性发热性嗜中性皮病中的表达情况；了解psoriasin在炎症性皮肤病中的作用；为明确psoriasin在疾病中的作用机制提供理论依据。方法：选取吉林大学第二医院皮肤科近16年（1996.06-2011.03）经临床及皮肤组织病理学明确诊断为扁平苔藓及急性发热性嗜中性皮病的石蜡包埋组织标本，应用免疫组织化学方法，观察psoriasin在上述疾病组织中的表达情况。结果： Psoriasin在正常皮肤组织中未见表达，在扁平苔藓中及急性发热性嗜中性皮病中均有阳性表达。将20例正常皮肤组织作为对照组，其中阳性0例，阴性20例。在30例扁平苔藓中，psoriasin阳性28例（93.33%），阴性2例（6.67%），，与对照组比较有显著性差异（p0.01）；在30例急性发热性嗜中性皮病中，psoriasin阳性26例（86.67%），阴性4例（13.33%），与对照组比较有显著性差异（p0.01）。结论： 1、首次研究了psoriasin在扁平苔藓、急性发热性嗜中性皮病的表达情况。 2、免疫组织化学染色结果显示psoriasin在扁平苔藓和急性发热性嗜中性皮病的表皮层均有阳性表达； 3、Psoriasin可能参与了扁平苔藓、急性发热性嗜中性皮病的发生、发展过程； 4、扁平苔藓皮损中CD4+T细胞主要在真皮层浸润，急性发热性嗜中性皮病中中性粒细胞主要在真皮层集聚，提示psoriasin不仅能参与角质层炎症反应，而且可能介导真皮层炎症细胞的趋化。
[Abstract]:Psoriasin, a member of the S100 protein family, was first found in psoriatic lesions in 1991. In the past 20 years, psoriasin has been found in many kinds of inflammatory scaly dermatosis and skin cancer, such as atopic dermatitis, mycosis fungoides and so on. It is not only related to the proliferation and differentiation of keratinocytes, but also has the biological function of chemotactic neutrophils and CD4 T cells to the cuticular layer. In recent years, it has also been found that psoriasin is involved in the antibacterial activity of the skin. The correlation between Psoriasin and dermatosis has been paid more and more attention, but the specific mechanism of Psoriasin in inflammatory dermatosis is not clear, and the effect of Psoriasin on dermis inflammation needs to be further studied. Objective: to investigate the expression of psoriasin in lichen planus and acute febrile neutral dermatosis, to understand the role of psoriasin in inflammatory dermatosis, and to provide theoretical basis for understanding the mechanism of psoriasin in the disease. Methods: paraffin embedded tissue specimens diagnosed as lichen planus and acute febrile neutral dermatosis were selected from the Department of Dermatology, the second Hospital of Jilin University in recent 16 years (June 06, 1996.2011.03), which were diagnosed as lichen planus and acute febrile neutral dermatosis. The expression of psoriasin in the above diseases was observed by immunohistochemistry. Results: Psoriasin was not expressed in normal skin tissue, but positive in lichen planus and acute febrile neutrophil disease. 20 cases of normal skin tissue were used as control group, of which 0 cases were positive and 20 cases were negative. In 30 cases of lichen planus, psoriasin was positive in 28 cases (93.33%) and negative in 2 cases (6.67%), which was significantly different from that in the control group (p0.01). In 30 cases of acute febrile neutral dermatosis, psoriasin was positive in 26 cases (86.67%) and negative in 4 cases (13.33%), which was significantly different from that in the control group (p0.01). Conclusion: 1. The expression of psoriasin in lichen planus and acute febrile neutrophil disease was studied for the first time. 2. 2. The results of Immunohistochemical staining showed that psoriasin was positive in the epidermis of lichen planus and acute febrile neutrophil disease. (3) Psoriasin may be involved in the occurrence and development of lichen planus and acute febrile neutrophil disease, and the expression of Psoriasin may be involved in the occurrence and development of lichen planus and acute febrile neutrophil disease. 4. CD4 T cells were mainly infiltrated in the dermis of lichen planus, and neutrophils of acute febrile neutrophils were mainly concentrated in the dermis, suggesting that psoriasin could not only participate in cuticular inflammation. It may also mediated the chemotaxis of inflammatory cells in dermis.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R758.6

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