mB7H4-Ig融合蛋白对咪喹莫特诱导小鼠银屑病样皮损的干预作用

发布时间：2019-07-31 11:10

【摘要】：目的:探讨mB7H4-Ig融合蛋白对咪喹莫特诱导小鼠银屑病的干预作用。方法:ELISA法测定高压注射重组质粒在体内的表达水平;用咪喹莫特诱导小鼠银屑病模型,治疗组高压注射mB7H4-Ig质粒而对照组注射Flag-Ig质粒,HE染色检测皮损改变,流式细胞术测定外周血免疫细胞亚群比例。结果:高压注射后小鼠体内可表达mB7H4-Ig融合蛋白;与对照组小鼠相比,治疗组小鼠皮肤的银屑病皮损炎症症状及病理改变相对较轻,PASI分数降低,外周血中的中性粒细胞比例明显降低而CD4~+T细胞比例上调。结论:mB7H4-Ig融合蛋白通过抑制炎症反应因而改善咪喹莫特诱导的小鼠银屑病皮损病变,有望成为治疗银屑病新策略。
[Abstract]:Objective: To study the effect of mB7H4-Ig fusion protein on the induction of psoriasis in mice. Methods: The expression level of high-pressure injected recombinant plasmid in the body was determined by ELISA. The model of mouse psoriasis was induced by immoterol. The control group was injected with mB7H4-Ig plasmid, and the control group was injected with Flag-Ig plasmid, and HE staining was used to detect the changes of the skin lesions. Flow cytometry was used to determine the proportion of immune cell subsets in peripheral blood. Results: The mB7H4-Ig fusion protein can be expressed in the mice after high-pressure injection; compared with the control group, the inflammation symptoms and the pathological changes of the psoriatic lesions in the treatment group are relatively light, the PASI score is reduced, the proportion of the neutrophils in the peripheral blood is obviously reduced, and the proportion of the CD4 + T cells is up-regulated. Conclusion: The mB7H4-Ig fusion protein is expected to be a new strategy for the treatment of psoriasis by inhibiting the inflammatory reaction and improving the lesion of the skin lesions of the mouse psoriasis induced by Mb7H4-Ig.
【作者单位】：福建医科大学免疫治疗研究所临床医学部;
【基金】：福建省自然科学基金(2017J01525) 福建省大学生创新创业训练计划项目(201610392058)
【分类号】：R758.63

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