三氧化二砷对神经母细胞瘤细胞Trk基因家族表达的影响

发布时间：2017-12-28 20:22

  本文关键词：三氧化二砷对神经母细胞瘤细胞Trk基因家族表达的影响　出处：《中华肿瘤防治杂志》2016年17期 　论文类型：期刊论文

  更多相关文章： 神经母细胞瘤 Trk基因家族 三氧化二砷 细胞周期 化疗

【摘要】：目的神经母细胞瘤(neuroblastoma,NB)是儿童常见的实体瘤,三氧化二砷(Arsenic troxide,As_2O_3)现已广泛应用于治疗实体肿瘤,为了探讨As_2O_3治疗NB的可行性,本研究检测As_2O_3对人SK-N-SH细胞周期及Trk基因家族表达的影响。方法通过流式细胞术检测4μmol/L浓度的As_2O_3作用48h后SK-N-SH细胞细胞周期的变化;qRT-PCR法检测As_2O_3在1、2和4μmol/L浓度下对SK-N-SH细胞分别作用24和48h后,对SK-N-SH细胞内TrkA、TrkB和TrkC基因表达的影响。结果用4μmol/L As_2O_3作用48h后,SK-N-SH细胞的G2/M期细胞比例为(30.93±0.34)%,而对照组G_2/M期细胞比例为(5.29±0.23)%,两组间差异有统计学意义,P0.01。1μmol/L As_2O_3作用SK-N-SH细胞24h的TrkA比值为1.43±0.17,48h为3.88±0.26,两者比较差异有明显统计学意义,P0.01,但随着As_2O_3浓度继续增大至4μmol/L TrkA反而下调。SK-N-SH细胞的TrkB及TrkC基因表达均随着As_2O_3浓度增加而上调,4μmol/L浓度下与对照组比较差异有统计学意义(P0.05),48h比24h上调更明显。结论初步研究结果表明,As_2O_3不仅对NB的SK-N-SH细胞有明显杀伤作用,使其阻滞在G_2/M期,还能使与NB预后密切相关的Trk基因的表达发生变化,As_2O_3有用于NB治疗的潜在临床价值。
[Abstract]:The purpose of neuroblastoma (neuroblastoma, NB) is a common solid tumor, arsenic trioxide (Arsenic troxide As_2O_3) has been widely used in the treatment of solid tumors, in order to evaluate the feasibility of As_2O_3 in the treatment of NB, this study detected the expression of As_2O_3 on SK-N-SH cell cycle and Trk gene family. The method was examined by flow cytometry. As_2O_3 48h 4 mol/L concentration after SK-N-SH cell cycle changes; detection of As_2O_3 qRT-PCR in 1, 2 and 4 mol/L concentration of SK-N-SH cells respectively 24 and 48h, on the expression of TrkA, TrkB and TrkC genes in SK-N-SH cells influence. Results after 4 48h mol/L treatment, the proportion of G2/M phase cells in SK-N-SH cells was (30.93 + 0.34)%, while in the control group, the proportion of G_2/M phase cells was (5.29 + 0.23)%. The difference between the two groups was statistically significant (P0.01). The proportion of G_2/M cells in the control group was (5.29 + 0.23)%. The TrkA ratio of 24h cells treated with 1 mu mol/L As_2O_3 was 1.43 + 0.17,48h, which was 3.88 + 0.26. The difference between them was statistically significant, P0.01, but decreased with increasing As_2O_3 concentration to 4 SK-N-SH mol/L TrkA. The expression of TrkB and TrkC genes in SK-N-SH cells increased with the increase of As_2O_3 concentration. There was a significant difference between the 4 mol/L concentration and the control group (P0.05), 48h increased more significantly than 24h. Conclusion the preliminary results indicate that As_2O_3 not only has obvious killing effect on NB SK-N-SH cells, but also blocks G_2/M phase, and also changes the expression of Trk gene, which is closely related to NB prognosis. As_2O_3 has potential clinical value for NB therapy.
【作者单位】： 中山大学孙逸仙纪念医院儿童血液/肿瘤专科;广东医学院附属医院儿童医学中心;中山大学生命科学学院;
【分类号】：R739.4
【正文快照】： 中华肿瘤防治杂志,2016,23(17):1149-1153Chin J Cancer Prev Treat,2016,23(17):1149-1153神经母细胞瘤(neuroblastoma,NB)是儿童时期最常见的颅外实体瘤之一,由于4期及难治性NB目前单凭化疗5年生存率仅30%左右[1],辅以自体造血干细胞移植后5年生存率才能达到50%[2]。三氧化，

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