脊神经结扎大鼠脊髓背角结构可塑性的体视学研究

发布时间：2018-01-05 09:46

本文关键词：脊神经结扎大鼠脊髓背角结构可塑性的体视学研究　出处：《川北医学院》2015年硕士论文　论文类型：学位论文

【摘要】：目的:探讨L5脊神经紧结扎(SNL)所致神经病理性疼痛(NP)对大鼠脊髓背角内神经元、突触、小胶质细胞及星形胶质细胞数量的影响。方法:将29只3月龄雄性SD(Sprague-Dawley)大鼠随机分为2组:SNL组(n=16,行单侧L5脊神经紧结扎)和假手术组(n=13,行单侧假手术)。每组分别于术后7天和28天切取L5节段脊髓进行石蜡包埋并制作14μm厚的连续切片50张,采用等距随机抽样原则分别各抽选3-5张用尼氏染色以及Synaptophysin、IBa-1、GFAP抗体的免疫组织化学染色显示神经元、突触素颗粒、小胶质细胞及星形胶质细胞。采用体视学新技术——光学体视框估计脊髓背角内神经元、突触、小胶质细胞、星形胶质细胞的数量的变化结果:与对侧未手术侧相比,SNL组手术侧术后7 d单位长度L5节段脊髓背角内的突触数及突触数与神经元数之比均无显著变化,小胶质细胞及星形胶质细胞数量显著增加了210%和62%;术后28 d,突触数及突触数与神经元数之比分别显著增加了29%和20%,小胶质细胞的数量显著增加了120%,星形胶质细胞数量无显著变化。与对侧未手术侧相比,假手术组手术侧神经元、突触、小胶质细胞和星形胶质细胞的数量术后7d和28 d均无显著性变化。SNL组手术侧术后28 d脊髓背角内的小胶质细胞数与星形胶质细胞数与7 d相比分别显著减少了48%和20%。结论:L5脊神经结扎导致大鼠脊髓背角发生结构上的可塑性变化,该变化随着NP的进展呈现出时间相关性的动态趋势:突触数量呈现由无显著增加到逐渐出现显著增加的变化趋势;胶质细胞数量则呈现早期极显著增加,随后逐渐减少的变化趋势。小胶质细胞和星形胶质细胞可能与外周神经损伤早期NP的诱导和产生有关,突触可能与NP的维持有关。
[Abstract]:Objective: To investigate the L5 spinal nerve tight ligation (SNL) induced neuropathic pain (NP) synapses on rat spinal cord dorsal horn neurons, and affect the number of microglia and astrocytes. Methods: 29 March old male SD (Sprague-Dawley) rats were randomly divided into 2 groups: SNL group (n=16. Unilateral tight ligation of L5 spinal nerve) and sham operation group (n=13, unilateral sham). Each group in 7 days and 28 days after the L5 segment of the spinal cord were embedded in paraffin and made 14 consecutive sections with the thickness of M 50, using a uniform random sampling principle, all selected by 3-5 Zhang Nissl staining and Synaptophysin, IBa-1, immunohistochemistry of GFAP antibody staining showed that synaptophysin granules, neurons, astrocytes and microglia. Using stereological technique -- optical disector estimation in the spinal dorsal horn neurons, synapses, microglial cells, the number of astrocytes The change results: compared with the contralateral side of surgery, the operative side in group SNL after 7 d per unit length of L5 segment of the spinal cord in the dorsal horn of the number and the number of synapses and synaptic neuron number ratio did not change significantly, the number of microglia and astrocytes was significantly increased by 210% and 62%; 28 d after operation the number, the number of synapses and synaptic and neuronal number ratio were increased by 29% and 20%, the number of microglia cells were significantly increased by 120%, the number of astrocytes. No significant changes compared with the contralateral unoperated side, sham operated group, neurons, synapses, the number of microglia and astrocytes. After 7d and 28 d were no significant changes in.SNL operated group after 28 d in the spinal dorsal horn microglia and astrocytes number compared with 7 d were significantly reduced by 48% and 20%. conclusion: L5 spinal nerve ligation in the rat spinal dorsal horn structure can occur The plastic changes, the changes with the progress of NP shows a dynamic trend time correlation: the number of synapses presented by no significant increase trend to gradually increased significantly; number of glial cells showed a significant increase trend in early stage, and then decreased gradually. Microglia and astrocytes and peripheral nerve the early damage induced by NP and related synapses may be related to NP.

【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R741

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