神经型一氧化氮合酶参与脂多糖诱导的大鼠脑微出血的相关性研究

发布时间：2018-01-20 09:47

本文关键词： 脑微出血脂多糖神经型一氧化氮合酶磁敏感加权成像血脑屏障　出处：《中风与神经疾病杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的改进并稳定脂多糖(LPS)诱导脑微出血(CMBS)动物模型的建立方法,为进一步研究提供稳定成熟的技术手段;探讨神经型一氧化氮合酶(nNOS)在CMBS过程中的作用。方法 40只SD大鼠,随机分成LPS给药组(n=20)和生理盐水对照组(n=20),分别于0 h、12 h和24 h腹腔注射1 mg/ml、3 mg/kg LPS或相同剂量的生理盐水,48 h后行头部MRI扫描,SWI序列显示出血灶;免疫荧光染色显示小胶质细胞标记分子Iba的表达;蛋白印迹法分析nNOS和ZO-1(血脑屏障标记分子)的表达情况。结果 3 mg/kg LPS给药后,MRI显示散在SWI序列点状低信号影,蛋白印迹法及免疫荧光结果显示ZO-1明显减少,Iba及nNOS表达显著增多。结论 LPS可能通过增加全身炎症反应,促进脑内小胶质细胞增殖,增加nNOS的表达,对中枢神经系统血脑屏障产生破坏作用,从而导致微出血的产生。
[Abstract]:Objective to improve and stabilize the animal model of intracerebral microhemorrhage induced by lipopolysaccharide (LPS), and to provide a stable and mature technique for further study. To investigate the role of neuronal nitric oxide synthase (nNOS) in the process of CMBS. Methods Forty Sprague-Dawley rats were used. The rats were randomly divided into LPS group (n = 20) and normal saline control group (n = 20). They were injected intraperitoneally for 1 mg/ml at 0 h, 12 h and 24 h, respectively. 3After 48 hours of mg/kg LPS or the same dose of normal saline, the bleeding foci were displayed by MRI scanning. Immunofluorescence staining showed the expression of microglial marker molecule Iba. The expression of nNOS and ZO-1 (blood-brain barrier marker) were analyzed by Western blotting. Results 3 mg/kg LPS showed dot hypo imaging of SWI sequence. The results of Western blot and immunofluorescence showed that ZO-1 significantly decreased the expression of IBA and nNOS. Conclusion LPS may promote the proliferation of microglia by increasing systemic inflammation. Increasing the expression of nNOS can destroy the blood-brain barrier of central nervous system and lead to microhaemorrhage.
【作者单位】：中国人民解放军陆军总医院神经内科;陕西中医药大学;北京四环制药有限公司;中国人民解放军第四五一医院神经内科;
【分类号】：R743.34
【正文快照】： 作者单位:(1.中国人民解放军陆军总医院神经内科,北京100700;2.陕西中医药大学,陕西咸阳712046;3.北京四环制药有限公司,北京101113;4.中国人民解放军第四五一医院神经内科,陕西西安710054)脑小血管病(cerebral small vessel disease,CS-VD)是指在多种病因共同作用下,导致脑的

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