糖尿病小鼠局灶性脑缺血再灌注损伤中脑周细胞的变化

发布时间：2018-01-22 02:32

本文关键词： 脑缺血再灌注高血糖周细胞 α-平滑肌肌动蛋白　出处：《安徽医科大学学报》2017年11期 　论文类型：期刊论文

【摘要】：目的探讨糖尿病高血糖状态下局灶脑缺血再灌注损伤中脑周细胞的变化特点。方法采用四氧嘧啶一次性腹腔注射制备1型糖尿病高血糖C57BL/6小鼠模型,以线栓法为基础使大脑中动脉缺血建立局灶性脑缺血再灌注小鼠模型,使用行为学、组织化学、qRT-PCR及Western blot等方法,对比观察假手术组(空白对照组)、正常血糖脑缺血再灌注组(缺血组)、糖尿病高血糖脑缺血再灌注组(高糖并缺血组)大脑皮质区周细胞数量改变和α-平滑肌肌动蛋白(α-SMA)的相对表达情况。结果免疫组化及TTC染色显示,与空白对照组比较,缺血组缺血1 h再灌注24 h后脑组织中α-SMA阳性细胞数量明显增多,梗死脑组织水肿明显(P0.05);高糖并缺血组再灌注24 h,α-SMA阳性细胞数目较同期缺血组更多,梗死区域水肿极严重(P0.05);qRT-PCR和Western blot分别显示高糖并缺血组α-SMA蛋白对应基因、α-SMA蛋白的相对表达量均高于缺血组(P0.05)。结论糖尿病高血糖与缺血再灌注两种损伤机制同时作用于脑组织时,使脑组织损伤更严重,损伤区域α-SMA的表达增强,周细胞数量明显增加。
[Abstract]:Objective to investigate the changes of pericyte in focal cerebral ischemia-reperfusion injury induced by diabetic hyperglycemia. Methods the model of type 1 diabetes with hyperglycemia C57BL / 6 was established by single intraperitoneal injection of alloxan. . The animal model of focal cerebral ischemia reperfusion was established by middle cerebral artery ischemia on the basis of thread embolization. Behavior, histochemical qRT-PCR and Western blot were used. The sham operation group (blank control group) and the normal blood glucose cerebral ischemia reperfusion group (ischemia group) were observed. Changes in the number of pericortex cells and 伪 -smooth muscle actin (伪 -SMA) in the hyperglycemic cerebral ischemia-reperfusion group (hyperglycemia with ischemia group). Results Immunohistochemistry and TTC staining showed. Compared with the blank control group, the number of 伪 -SMA positive cells in cerebral tissue of ischemic group increased significantly after 1 h of ischemia and 24 h of reperfusion, and the edema of infarcted brain tissue was significantly increased (P 0.05). After 24 h reperfusion, the number of 伪 -SMA positive cells in high glucose with ischemia group was more than that in ischemia group, and the edema in infarct area was very serious (P 0.05). QRT-PCR and Western blot showed 伪 -SMA protein corresponding genes in hyperglycemia and ischemia group respectively. The relative expression of 伪 -SMA protein was higher than that of ischemia group (P 0.05). Conclusion the hyperglycemia and ischemia-reperfusion injury mechanism in the brain tissue at the same time make the brain tissue damage more serious. The expression of 伪 -SMA was increased and the number of pericytes was increased.
【作者单位】：安徽医科大学第二附属医院神经外科;安徽医科大学药学院;
【基金】：国家自然科学基金(编号:31540021)
【分类号】：R587.2;R743
【正文快照】： 周细胞是血管神经单元的重要组成成分之一,广泛分布于全身各组织如脑、心脏、眼睛等的微血管和毛细血管壁而被微血管基底膜包被,位于内皮细胞外,其具有多种功能并在脑部参与血脑屏障功能的维持[1-2]。α-平滑肌肌动蛋白(α-smooth muscleactin,α-SMA)是微丝的线性结构蛋白,是

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