电针刺激联合BMSCs移植对脑出血大鼠出血灶周边区水通道蛋白4表达的影响

发布时间：2018-01-24 12:55

本文关键词： 脑出血电针刺激骨髓间充质干细胞水通道蛋白　出处：《中国临床解剖学杂志》2016年06期 　论文类型：期刊论文

【摘要】：目的探讨骨髓间充质干细胞(BMSCs)移植与电针刺激联合治疗对脑出血大鼠出血灶周边区水通道蛋白4(AQP-4)表达的影响。方法向成年SD大鼠右侧尾状核注射肝素和胶原酶诱导脑出血,并于术后第3天向出血灶移植BMSCs后施以电针刺激(Ea-BMSCs组),同时设置BMSCs移植组(BMSCs组)、生理盐水移植组(Control组)和电针刺激组(Ea组)。各组分别于治疗后1、3、5、7和14 d收集脑组织标本,采用干湿法检测脑组织水含量,免疫组化和Western Blotting检测出血灶周边区AQP-4的表达变化。采用单因素方差LSD法进行统计分析。结果 1 AQP-4表达在软脑膜、大脑皮质、侧脑室脉络组织以及出血灶周边脑区,且与GFAP共表达于星形胶质细胞上。2各实验组脑组织水含量在第3天开始升高,第5天达峰值,随后逐渐降低。3各组AQP-4蛋白在第3天开始升高,第5天达峰值,Control组和Ea组在达峰值后未见升高,而BMSCs组和Ea-BMSCs组在第14天再度出现峰值。4在第5天和第7天时,Ea-BMSCs组、BMSCs组AQP-4表达均低于Control组(P0.05),Ea-BMSCs组与BMSCs组之间表达无差异;在第14天时,Ea-BMSCs组AQP-4表达高于Control组和BMSCs组(P0.05)。结论电针刺激联合BMSCs移植可显著降低脑组织水含量,可能与电针刺激使移植后表达上调的AQP-4活化发挥水转运功能有关。
[Abstract]:Objective to investigate the effects of bone marrow mesenchymal stem cells (BMSCs) transplantation combined with electroacupuncture stimulation on aquaporin-4 aquaporin-4 (AQP-4) in the peripheral area of intracerebral hemorrhage in rats with intracerebral hemorrhage (ICH). Methods intracerebral hemorrhage was induced by injection of heparin and collagenase into the right caudate nucleus of adult SD rats. On the third day after operation, BMSCs was transplanted to bleeding focus and electroacupuncture was used to stimulate the Ea-BMSCs group, and the BMSCs transplantation group was set up at the same time. Normal saline transplantation group (control group) and electroacupuncture stimulation group (EA group) were collected on the 7th and 14th day after treatment. The content of water in brain tissue was measured by dry-wet method. Immunohistochemistry and Western Blotting were used to detect the expression of AQP-4 in the peripheral region of bleeding foci. Single factor variance LSD method was used to analyze the expression of AQP-4. Results 1. AQP-4 was expressed in pial meninges. The contents of water in cerebral cortex, choroid tissue of lateral ventricle and cerebral area around hemorrhage foci, and co-expressed with GFAP in astrocytes, began to increase on the 3rd day and reached the peak on the 5th day. The protein of AQP-4 began to increase on the 3rd day, and on the 5th day, the protein of AQP-4 in the control group and EA group did not increase after reaching the peak value. On the 14th day, the peak value of Ea-BMSCs was found again in the BMSCs group and the Ea-BMSCs group on the 5th and 7th day. The expression of AQP-4 in BMSCs group was lower than that in Control group. There was no difference between Ea-BMSCs group and BMSCs group. On the 14th day. The expression of AQP-4 in Ea-BMSCs group was higher than that in Control group and BMSCs group (P 0.05). Conclusion electroacupuncture combined with BMSCs transplantation can significantly reduce the water content in brain tissue. It may be related to the effect of electroacupuncture stimulation on the up-regulation of AQP-4 expression after transplantation.
【作者单位】：西南医科大学人体解剖学教研室;西南医科大学神经生物研究室;郑州大学人体解剖学教研室;
【基金】：四川省应用基础研究计划项目(2013JY0075) 四川省教育厅重点项目(12ZA077)
【分类号】：R743.34
【正文快照】： 3.郑州大学人体解剖学教研室,郑州450001Corresponding author:YANG Chao-xian,E-mail:lyycx@hotmail.com脑出血(Intracerebral hemorrhage,ICH)是神经系统常见急症,发病率和死亡率相当高。脑出血发生后,多种病理因素综合作用引发严重脑水肿,导致神经元死亡并阻碍神经修复,使

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