神经酸对帕金森病模型小鼠运动障碍的缓解作用及机制研究

发布时间：2018-02-02 15:18

本文关键词： 神经酸帕金森病小鼠运动障碍多巴胺　出处：《中国药房》2017年19期 　论文类型：期刊论文

【摘要】：目的:研究神经酸对帕金森病(PD)模型小鼠运动障碍的缓解作用及机制。方法:将小鼠随机分为空白对照组(生理盐水)、模型组(生理盐水)、多巴丝肼片组(阳性对照,按左旋多巴计120 mg/kg)和神经酸低、中、高剂量组(20.0、40.0、80.0 mg/kg),每组10只。除空白对照组外,其余各组小鼠均建立PD模型。成模后,每天ig相应药物1次,连续14 d。末次给药后,观察各组小鼠行为学变化,采用高效液相色谱法测定小鼠脑纹状体内多巴胺(DA)及其代谢物二羟苯乙酸(DOPAC)、高香草酸(HVA)的含量。结果:与空白对照组比较,模型组小鼠爬杆时间延长、滚筒时间缩短、自发运动次数减少(P0.05),脑纹状体内DA、DOPAC、HVA含量均降低(P0.05)。与模型组比较,多巴丝肼片组和神经酸各剂量组小鼠爬杆时间缩短、滚筒时间延长(P0.05),脑纹状体内DA、DOPAC、HVA含量均升高(P0.05);多巴丝肼片组和神经酸高剂量组小鼠自发运动次数增加(P0.05)。结论:神经酸可有效改善PD模型小鼠的运动障碍症状,其机制可能与增加脑纹状体内DA含量有关。
[Abstract]:Objective: to study the effect and mechanism of nerve acid on motor disorder in PD mice. Methods: the mice were randomly divided into control group (normal saline group) and model group (normal saline group). Dobutylhydrazine group (positive control, 120 mg / kg L-dopa) and low, high dose group (20.0 ~ 40.0 mg 路kg ~ (-1)). PD model was established in all mice except the blank control group. After the model was established, the mice were given the corresponding drugs once a day for 14 days, and the behavioral changes of each group were observed. High performance liquid chromatography (HPLC) was used to determine the contents of dopaminergic acid (DAA) and its metabolites (DOPAC, HVA) in the striatum of mice. Results: compared with the blank control group, the contents of dopamine (DA) and dihydroxyphenylacetate (DOPA) were determined by high performance liquid chromatography. In the model group, the time of climbing rod was prolonged, the time of roller was shortened, the number of spontaneous movement was decreased (P 0.05), and the DAA DOPAC in the striatum was decreased. Compared with the model group, compared with the model group, the mice in the dobutazide group and the nerve acid group had shorter climbing time, longer roller time and DA in the striatum. The content of HVA in DOPAC was increased (P 0.05). The number of spontaneous exercise increased in dobutylhydrazine group and high dose group. Conclusion: neuronal acid can effectively improve the symptoms of motor disorder in PD model mice. The mechanism may be related to the increase of DA content in the striatum.
【作者单位】：枣庄科技职业学院医学技术系;山东益康药业股份有限公司山东省晶型药物重点实验室;
【分类号】：R-332;R742.5
【正文快照】： *讲师,硕士。研究方向:新药开发。电话:0632-5953070。E-mail:zhenghui198334@163.com帕金森病(Parkinson’s disease,PD)为一种常见的神经系统退行性疾病,临床表现为静止震颤、肌肉强直、自发运动障碍等症状,患者生活自理能力较差。中国现有PD患者约170万人,并保持每年约6%的

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