长春西汀减轻脑出血大鼠脑组织的炎症损伤

发布时间：2018-02-09 11:20

本文关键词： 长春西汀脑出血 Toll样受体核因子κB 黏附分子　出处：《中国病理生理杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的:探讨长春西汀对脑出血大鼠的干预作用以及对炎症损伤的影响。方法:将大鼠随机分为假手术组、脑出血组以及长春西汀低剂量组、中剂量组和高剂量组。除假手术组外,其余大鼠均通过注射VII型胶原酶的方法建立脑出血模型,长春西汀低剂量组、中剂量组和高剂量组分别给予腹腔注射0.5、1.0和1.5 mg/kg长春西汀,每天1次,共给药7 d。给药完成后,对各组大鼠进行神经功能缺损评分,称重法计算脑组织的含水量,检测脑组织中髓过氧化物酶(MPO)活性,Western blot法检测脑组织中Toll样受体4(TLR4)、核因子κB(NF-κB)、细胞间黏附分子1(ICAM-1)与血管细胞黏附分子1(VCAM-1)的蛋白表达。结果:与脑出血组相比,给予长春西汀干预的大鼠神经功能缺损评分显著下降(P0.05),脑组织含水量明显下降(P0.05),MPO活性显著降低(P0.05),脑组织中TLR4、NF-κB、ICAM-1与VCAM-1的蛋白表达显著下降(P0.05)。结论:长春西汀对脑出血大鼠脑组织具有保护作用,可能是通过抑制TLR4诱导的NF-κB信号通路以及ICAM-1与VCAM-1的表达来抑制炎症反应。
[Abstract]:Objective: to investigate the effects of vinpocetine on cerebral hemorrhage and inflammatory injury in rats. Methods: rats were randomly divided into sham-operation group, intracerebral hemorrhage group and vinpocetine low-dose group. Except sham-operation group, the other rats were injected with VII collagenase to establish intracerebral hemorrhage model. Vinpocetine low dose group, middle dose group and high dose group were intraperitoneally injected with vinpocetine for 0.5 and 1.5 mg/kg, respectively. Once a day for 7 days, the rats in each group were evaluated for neurological deficit, and the water content of brain tissue was calculated by weighing method. The activity of myeloperoxidase (MPO) in brain tissue was detected by Western blot. The protein expression of Toll like receptor 4 (TLR4), nuclear factor 魏 B (NF- B) NF- 魏 B, intercellular adhesion molecule 1 (ICAM 1) and vascular cell adhesion molecule 1 (VCAM-1) in brain tissue was detected by Western blot. The neurologic impairment score of rats treated with vinpocetine decreased significantly (P0.05A), the water content of brain tissue decreased significantly (P0.05), the activity of MPO decreased significantly (P0.05), and the protein expression of TLR4NF- 魏 Bmc-ICAM-1 and VCAM-1 decreased significantly in brain tissue. Conclusion: vinpocetine can treat intracerebral hemorrhage. The rat brain has protective effect. TLR4 induced NF- 魏 B signaling pathway and the expression of ICAM-1 and VCAM-1 may inhibit the inflammatory response.
【作者单位】：河北医科大学第二医院神经外科;河北中医学院护理学院;河北中医学院实验中心;河北中医学院基础医学院;
【基金】：河北省医学科学研究重点课题计划项目(No.ZD20140110;No.ZD20150221) 河南润弘制药股份有限公司赞助的横向项目
【分类号】：R743.34

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