超声联合微泡增效骨髓间充质干细胞归巢治疗缺血性脑卒中

发布时间：2018-02-11 06:38

本文关键词： 脑缺血骨髓间质干细胞移植微气泡超声处理组织工程干细胞移植超声微泡骨髓间充质干细胞缺血性脑卒中归巢　出处：《中国组织工程研究》2017年25期 　论文类型：期刊论文

【摘要】：背景:国内外动物实验结果表明,超声微泡能够促进移植干细胞归巢到缺血区,增强血管新生和小动脉生成,改善缺血心肌局部血流量,恢复心肌收缩功能。目的:探讨超声联合微泡对静脉移植骨髓间充质干细胞归巢的影响及治疗缺血性脑卒中的有效性。方法:插线法建立大脑中动脉栓塞大鼠模型,建模72 h后,随机分为4组:PBS组(n=15),骨髓间充质干细胞组(n=18),超声+骨髓间充质干细胞组(n=18),超声+微泡+骨髓间充质干细胞组(n=18)。PBS组经尾静脉注射2 mL PBS;骨髓间充质干细胞组将骨髓间充质干细胞(约3×10~6)用2 mL PBS稀释后经尾静脉缓慢注射;超声+骨髓间充质干细胞组经颅骨超声(频率1 MHz,功率2 W/cm~2)辐照120 s,给予骨髓间充质干细胞;超声+微泡+骨髓间充质干细胞组在超声辐照前静注微泡对比剂0.1 mL/kg,再给予骨髓间充质干细胞。结果与结论:(1)移植后48 h,超声+微泡+骨髓间充质干细胞组细胞的脑内归巢率高于骨髓间充质干细胞组和超声+骨髓间充质干细胞组,差异均有显著性意义(P0.05);(2)造模后28 d,超声+微泡+骨髓间充质干细胞组的神经损害程度较其余组轻,差异均有显著性意义(P0.05);(3)移植后7 d,超声+微泡+骨髓间充质干细胞组的脑组织含水率较其余组低,差异均有显著性意义(P0.05);(4)移植后7 d,超声+微泡+骨髓间充质干细胞组脑梗死体积较其余组小,差异有显著性意义(P0.05);(5)结果表明,超声联合微泡可促进骨髓间充质干细胞的脑内靶向归巢,能够减少脑水肿和脑梗死体积,改善神经功能评分,增效骨髓间充质干细胞移植治疗缺血性脑卒中的效果。
[Abstract]:Background: the results of animal experiments at home and abroad show that ultrasound microbubbles can promote the homing of stem cells to ischemic areas, enhance angiogenesis and arteriogenesis, and improve the local blood flow of ischemic myocardium. Objective: to investigate the effect of ultrasound combined with microbubbles on homing of bone marrow mesenchymal stem cells (BMSCs) after vein transplantation and the efficacy in the treatment of ischemic stroke. 72 hours after modeling, They were randomly divided into 4 groups: PBS group (n = 15), bone marrow mesenchymal stem cell group (n = 18), ultrasound bone marrow mesenchymal stem cell group (n ~ (18)), ultrasound microbubble bone marrow mesenchymal stem cell group (n ~ (18)) and PBS group (n = 2 mL) were injected with 2 mL PBSvia tail vein; bone marrow mesenchymal stem cell group (BMSCs) was injected with bone marrow. Mesenchymal stem cells (3 脳 10 6) were diluted with 2 mL PBS and injected slowly through the tail vein. Bone marrow mesenchymal stem cells (BMSCs) were irradiated with ultrasound for 120 s (frequency 1 MHz, power 2 W / cm 2) for 120 s, and bone marrow mesenchymal stem cells (BMSCs) were given. Ultrasound microbubble bone marrow mesenchymal stem cells group was given bone marrow mesenchymal stem cells by intravenous injection of 0.1 mL / kg contrast agent before ultrasound irradiation. Results and conclusion 48 hours after transplantation, ultrasound microbubble bone marrow mesenchymal stem cells were treated with ultrasound microbubble bone marrow mesenchymal stem cells. The homing rate in brain was higher than that in bone marrow mesenchymal stem cells group and ultrasound bone marrow mesenchymal stem cell group. There was significant difference between the two groups at 28 days after the model was made, the nerve damage in the ultrasound microbubble bone marrow mesenchymal stem cells group was lighter than that in the other groups. At 7 days after transplantation, the water content of brain tissue in the ultrasound microbubble bone marrow mesenchymal stem cells group was lower than that in the other groups. At 7 days after transplantation, the volume of cerebral infarction in the ultrasound microbubble bone marrow mesenchymal stem cells group was smaller than that in the other groups, and the difference was significant (P 0.05). Ultrasound combined with microbubbles can promote the homing of bone marrow mesenchymal stem cells, reduce the volume of brain edema and cerebral infarction, improve the neurological function score, and enhance the effect of bone marrow mesenchymal stem cell transplantation in the treatment of ischemic stroke.
【作者单位】：南京大学医学院附属鼓楼医院急诊科;
【基金】：南京市医学科技发展项目基金(YKK16100) 江苏省卫生厅青年科研课题(Q201306)~~
【分类号】：R743.3

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