颈内动脉刺破法制作大鼠蛛网膜下腔出血模型

发布时间：2018-02-11 22:49

本文关键词： 蛛网膜下腔出血血管穿刺法模型大鼠纤芯　出处：《中国比较医学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的通过图文结合的形式,详细介绍大鼠蛛网膜下腔出血模型的制作方法及过程;改进并创新模型,使其能更好的模拟临床动脉瘤破裂所致蛛网膜下腔出血。方法选取SD雄性大鼠,采用颈内动脉颅外段穿入颈内段法,建立SD雄性大鼠蛛网膜下腔出血模型,用呼吸、瞳孔、大小便及留置纤芯解剖大鼠头颈等方法对模型进行验证。通过观察死亡率和临床表现,探讨模型的可控性、可重复性。结果实现了刺破血管造成蛛网膜下腔出血,且纤芯到达的位置能准确的刺破所需要的血管。结论此法制作模型稳定,方法通俗易懂,操作精巧灵活,可重复性强,理解后短时间内就可造模成功,且成功造模所形成的蛛网膜下腔出血,能更好地模拟临床动脉瘤突然破裂出血,非常适用于蛛网膜下腔出血早期脑损害及其血管痉挛的研究。
[Abstract]:Objective to introduce the method and process of making rat model of subarachnoid hemorrhage, and to improve and innovate the model. Methods Sprague-Dawley male rats were used to establish the model of subarachnoid hemorrhage in male SD rats by penetrating the extracranial segment of the internal carotid artery into the internal segment of the neck, and breathing and pupillary were used to establish the model of subarachnoid hemorrhage in SD male rats. By observing the mortality and clinical manifestations of the model, the controllability and repeatability of the model were discussed. Results Subarachnoid hemorrhage caused by puncture of blood vessels was achieved. And the position reached by the core can accurately puncture the needed blood vessels. Conclusion the model of this method is stable, the method is easy to understand, the operation is clever and flexible, the repeatability is strong, and the model can be made successfully in a short time after understanding. The subarachnoid hemorrhage caused by successful modeling can better simulate the sudden rupture and hemorrhage of clinical aneurysms, which is very suitable for the study of cerebral damage and vasospasm in the early stage of subarachnoid hemorrhage.
【作者单位】：华北理工大学附属医院神经外科;遵化市人民医院神经外科;华北理工大学附属医院神经内科;华北理工大学基础医学院河北省慢性疾病重点实验室唐山市慢性病临床基础研究重点实验室;
【基金】：河北省省级重大医学项目(Zd2013093)
【分类号】：R-332;R743.35

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