钾通道阻断剂对胶质瘤细胞迁移和侵袭的影响

发布时间：2018-02-12 08:48

本文关键词： 钾通道神经胶质瘤迁移侵袭阻断剂　出处：《江西师范大学学报(自然科学版)》2017年02期 　论文类型：期刊论文

【摘要】：为了检测电压门控钾通道阻断剂4-氨基吡啶(4-AP)、四乙胺(TEA)和ATP敏感钾通道阻断剂格列苯脲(Glibenclamide,Gli)对胶质瘤细胞迁移和侵袭的影响,选用人胶质瘤细胞系U87和U251,其中钾通道阻断剂4-AP、TEA及Gli处理作为实验组,未处理的作为对照组.采用划痕实验和Transwell小室法检测钾通道阻断剂对U87和U251细胞迁移和侵袭能力的影响;Western blot检测药物处理后细胞高迁移率蛋白B1(high mobility group protein B1,HMGB1)表达水平.结果表明:5 mmol·L~(-1)的4-AP、40 mmol·L~(-1)的TEA及400μmol·L~(-1)的Gli可以显著抑制胶质瘤细胞的迁移、侵袭,并降低HMGB1表达水平.电压门控钾通道和ATP敏感钾通道对胶质瘤细胞迁移和侵袭具有重要调控作用,3种钾通道阻断剂对胶质瘤细胞迁移和侵袭有不同程度的抑制作用,可能通过调控HMGB1相关通路实现.
[Abstract]:To investigate the effects of voltage-gated potassium channel blockers 4-aminopyridine 4-APN, tetraethylamine tea) and ATP sensitive potassium channel blocker glibenclamide on the migration and invasion of glioma cells. Human glioma cell lines U87 and U251 were treated with potassium channel blocker 4-APTEA and Gli. The effect of potassium channel blocker on the migration and invasion of U87 and U251 cells was detected by scratch test and Transwell chamber assay. Western blot was used to detect the expression of high mobility protein B1, high mobility group protein B1, HMGB1) in U87 and U251 cells. The results showed that TEA of 40 mmol 路L ~ (-1) and Gli of 400 渭 mol 路L ~ (-1) could significantly inhibit the migration of glioma cells. Voltage-gated potassium channels and ATP sensitive potassium channels play important roles in regulating the migration and invasion of glioma cells. Three potassium channel blockers have different inhibitory effects on the migration and invasion of glioma cells. This may be achieved by regulating HMGB1 related pathways.
【作者单位】：江汉大学武汉生物医学研究院;
【基金】：国家自然科学基金青年科学基金(81302203)资助项目
【分类号】：R739.41

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