大鼠坐骨神经损伤后Spastin表达变化的实验研究

发布时间：2018-02-16 18:29

本文关键词： Spastin 坐骨神经神经损伤神经再生大鼠　出处：《中国修复重建外科杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的观察内源性Spastin蛋白在大鼠坐骨神经损伤后再生过程中的表达变化,探讨其在周围神经再生过程中的作用和意义。方法取成年雄性SD大鼠36只,随机分为实验组(n=30)和假手术对照组(n=6),实验组建立坐骨神经挤压损伤模型,对照组仅暴露坐骨神经。实验组分别于术后1、3、7、14、28 d(n=6),假手术组于术后7 d取大鼠坐骨神经相应节段的L4~6脊髓组织,采用实时荧光定量PCR检测Spastin m RNA相对表达量,Western blot检测Spastin蛋白表达;并取损伤远端5 mm处神经组织,通过透射电镜观察坐骨神经远端轴突的超微结构变化。结果两组大鼠L4~6节段脊髓组织中Spastin蛋白表达和基因表达变化趋势基本一致。实验组Spastin蛋白和基因表达量呈先下降后逐渐上升的趋势,于术后7 d降至最低,28 d时达初始水平。其中实验组术后3、7、14 d Spastin蛋白和基因表达量显著低于假手术对照组(P0.05);术后1、28 d与假手术对照组比较差异无统计学意义(P0.05)。实验组术后3、7、14 d Spastin蛋白和基因表达量显著低于术后1 d和28 d(P0.05),术后1 d和28 d比较差异无统计学意义(P0.05)。透射电镜观察发现实验组术后1、3、7 d,坐骨神经损伤远端髓鞘严重破坏;术后14 d雪旺细胞增生;术后28 d可见大量有髓神经纤维,接近正常形态。结论内源性Spastin蛋白在坐骨神经损伤后再生过程中出现了表达变化,提示Spastin可能在周围神经损伤后的再生过程中起着一定作用。
[Abstract]:Objective to investigate the expression and significance of endogenous Spastin protein in the regeneration of sciatic nerve after sciatic nerve injury in rats. Methods 36 adult male Sprague-Dawley rats were selected. The experimental group was randomly divided into two groups: the experimental group and the sham-operation control group. The experimental group was used to establish the sciatic nerve crush injury model. The sciatic nerve was only exposed in the control group. In the experimental group, the relative expression of Spastin m RNA was detected by real-time quantitative PCR and the expression of Spastin protein was detected by Western blot in the spinal cord of the corresponding segment of the sciatic nerve in the sham-operated group on the 7th day after operation. The nerve tissue at the distal end of the injury was taken. The ultrastructural changes of distal sciatic nerve axons were observed by transmission electron microscope. Results the changes of Spastin protein and gene expression in L4 and 6 segments of spinal cord of the two groups were basically the same. The expression of Spastin protein and gene in the experimental group was similar. The tendency to decline first and then rise gradually, The expression of Spastin protein and gene in the experimental group was significantly lower than that in the sham operation control group (P 0.05) on the 7th day after operation, but there was no significant difference between the control group and the sham operation control group on the 128 days after operation (P 0.05). The expression of Spastin protein and gene in the test group was significantly lower than that on the 1st and 28th day after operation (P 0.05). The results of transmission electron microscopy showed that the distal myelin sheath of the sciatic nerve injury was severely damaged in the experimental group at 1: 3 and 7 days after operation. On the 14th day after operation, Schwann cells proliferated, and 28 days after operation, a large number of myelinated nerve fibers were observed, which were close to normal morphology. Conclusion the expression of endogenous Spastin protein changes during the regeneration of sciatic nerve after injury. The results suggest that Spastin may play a role in the regeneration of peripheral nerve after injury.
【作者单位】：上海长征医院骨创伤与修复重建外科;
【基金】：上海市教委曙光计划项目(15SG34)~~
【分类号】：R745

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