桂西壮族人群RAAS相关基因多态性与CISS亚型相关性研究

发布时间：2018-02-26 04:31

本文关键词： 脑梗死肾素-血管紧张素-醛固酮系统基因多态性 CISS分型　出处：《广西医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的本研究拟探讨肾素-血管紧张素-醛固酮系统(renin-angiotension-aldosterone system, RAAS)相关基因多态性与桂西壮族人群按中国缺血性卒中亚型分型(Chinese Ischemic Stroke Subclassification, CISS)的脑梗死亚型的相关性,从遗传学角度研究脑血管病变的发病机制。方法采用聚合酶链反应(polymerase chain reaction, PCR)和基因测序技术对193例桂西壮族脑梗死患者和169例对照组的ACE、AGT、ATGR1及CYP11B2进行基因分型,根据CISS分型法将193例脑梗死患者分成大动脉粥样硬化(large artery atherosclerosis, LAA)组、穿支动脉疾病(penetracing artery disease, PAD)组、心源性(cardiogenic stroke, CS)组、其他原因(oetiologies, OE)组和不明确病因(undetermined etiology,UE)组。通过多因素logistics回归分析这四种基因与桂西壮族人群脑梗死各亚型的相关性。结果本对照研究共纳入桂西壮族脑梗死患者193例以及169例健康体检者对照。LAA组中ACE基因DD基因型(χ2=6.4036,P=0.047)和D等位基因(χ2=6.8803,P=-0.0087)频率高于对照组,AGT基因TT基因型(χ2=13.5614,P=0.0011)和T等位基因(χ2=4.2823,P=0.0385)频率明显高于对照组。PAD组和CS组中的AGTR1基因AC基因型(分别为χ2=4.0641,P=0.0438；x2=4.9288,P=0.0260)和C等位基因(分别为x2=3.9129,P=-0.0479；x2=4.7864,P=0.0287)频率高于对照组。五组脑梗死亚型中CYP11B2基因-344C/T TT基因型和T等位基因频率与对照组无显著性差异(P0.05)。多因素logistics回归显示LAA亚型中携带AGT基因TT基因型及携带ACE基因D等位基因可增加脑梗死风险分别为2.513倍(0R=2.513,95%CI：1.209～5.225)和1.702倍(OR=1.702,95%CI:1.012～2.863)。结论1.在桂西壮族人群中,RAAS相关位点基因多态性与脑梗死相关,RAAS相关基因中ACE基因D等位基因与AGT基因M235T TT基因型可能是大动脉粥样硬化亚型脑梗死的遗传易感基因。2.本研究未发现RAAS相关基因与脑梗死CS、CS、OE、UE亚型患者有相关性。
[Abstract]:Objective to investigate the relationship between gene polymorphism of renin-angiotension-aldosterone system (RAAS-Ras) and the subtype of Chinese Ischemic Stroke subtype (CISS) in Guanxi Zhuang population according to the subtype of ischemic stroke in China. Methods ACE-AGTATGR1 and CYP11B2 were genotyped by polymerase chain reaction (PCR) and gene sequencing in 193 patients with cerebral infarction in Guanxi Zhuang nationality and 169 controls. According to CISS classification, 193 patients with cerebral infarction were divided into three groups: large artery atherosclysis group, perforating artery disease artery disease group and cardiogenic cardiogenic stroke-group. The correlation between the four genes and the subtypes of cerebral infarction in the Zhuang nationality population in western Guangxi was analyzed by multivariate logistics regression analysis. Results this control study included 193 patients with cerebral infarction in western Guangxi. The frequencies of DD genotype and D allele of ACE gene in LAA group were significantly higher than those in control group (蠂 2 + 6.8803) and T allele (蠂 2 + 13.5614) and T allele (蠂 2 4.2823) respectively. The frequencies of DD genotype and D allele were significantly higher than those in control group and CS group. The frequencies of AC genotype (蠂 2 + 4.0641) and C allele (x 23.9129) were higher than those in the control group (蠂 ~ 2 + 4.0641). There was no significant difference in the frequency of CYP11B2 gene -344CTT genotype and T allele between the five groups and the control group (P 0.05). The multivariate logistics regression analysis showed that there was no significant difference in the frequency of CYP11B2 gene -344CTT genotype and T allele between the five groups and the control group (P < 0.05). The multivariate logistics regression analysis showed that there was no significant difference in the frequency of CYP11B2 gene -344CTT genotype and T allele between the five groups and the control group. The results showed that carrying TT genotype of AGT gene and D allele of ACE gene in LAA subtype could increase the risk of cerebral infarction. The risk of cerebral infarction was increased by 2.513 times and 1.702 times than 1.209% and 1.702 times respectively. Conclusion\\\;\\\%\%??? The D allele of the ACE gene and the M235T TT genotype of the AGT gene may be the genetic susceptibility genes of the cerebral infarction subtype of atherosclerosis. This study has not found the correlation between the RAAS related gene and the patients with the subtype of cerebral infarction.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R743.3

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