BAFF和BAFFR在难治性癫痫患者及大鼠模型中的表达变化

发布时间：2018-02-26 15:16

本文关键词： BAFF和BAFFR 颞叶癫痫免疫炎症反应　出处：《重庆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:BAFF主要通过调控BAFFR受体参与中枢神经系统免疫炎症反应的病理生理过程。目前尚不清楚BAFF和BAFFR是否也参与了癫痫的病理过程中。本研究主要通过对难治性癫痫患者以及癫痫大鼠模型脑组织中BAFF和BAFFR的表达变化,探讨BAFF和BAFFR在癫痫发病机制的作用。方法:1.本实验以24例难治性癫痫患者手术切除的颞叶病灶标本为实验组,以8例脑外伤患者手术切除的颞叶皮质作为对照组。2.将30只SD雄性大鼠随机均分为实验组和对照组,实验组SD大鼠给予匹鲁卡品腹腔注射,对照组给予生理盐水腹腔注射,观察癫痫动物模型的急性发作情况及2个月后的慢性发作情况。3.采用免疫荧光和免疫组化对BAFF和BAFFR进行蛋白表达定位,Western-blot和RT-qPCR技术对BAFF和BAFFR进行到蛋白表达定量。结果:免疫荧光结果显示BAFF和BAFFR在癫痫患者和大鼠模型神经元的细胞质和细胞膜中表达,在神经胶质细胞中不表达;免疫组化结果显示BAFF和BAFFR蛋白在癫痫患者及动物模型神经元的细胞质和细胞膜中都有表达,并且表达强度明显高于对照组(P0.05);Western-blot和RT-PCR结果显示BAFF和BAFFR在癫痫患者及动物模型中表达量皆较对照组明显升高(P0.05)。结论:BAFF和BAFFR在癫痫患者及癫痫大鼠模型中表达量明显升高。目前研究表明BAFF和BAFFR参与中枢神经系统的免疫及炎症反应,故我们推测升高的BAFF和BAFFR是通过免疫炎症反应来调节癫痫的发病过程并具有一定的作用。
[Abstract]:Objective: BAFF mainly through the pathophysiological process of regulation of BAFFR receptor in the central nervous system immune inflammation. It is unclear whether BAFF and BAFFR are involved in the pathophysiology of epilepsy. Through the research on the expression of patients with intractable epilepsy and epilepsy rat model of brain tissue of BAFF and BAFFR, to explore the the role of BAFF and BAFFR in the pathogenesis of epilepsy. Methods: 1. in this experiment, 24 cases of refractory epilepsy surgery in patients with temporal lobe lesions were chosen as experimental group, 8 patients with temporal lobe resection in patients with traumatic brain injury as the control group.2. 30 SD male rats were randomly divided into experimental group and control group, the experimental group SD rats were given intraperitoneal injection of pilocarpine, the control group was given intraperitoneal injection of normal saline, acute attack were observed in animal model of epilepsy and 2 months after the onset of chronic.3. by immunofluorescence and free Immunohistochemistry of BAFF and BAFFR protein expression, Western-blot and RT-qPCR technology of BAFF and BAFFR protein expression. Results: quantitative immunofluorescence showed that the expression of BAFF and BAFFR in neurons of rat model of epilepsy and the cytoplasm and cell membrane, no expression in glial cells; immunohistochemistry BAFF and BAFFR protein were expressed in neurons of epileptic patients and animal models of the cytoplasm and cell membrane, and the expression intensity was significantly higher than the control group (P0.05); Western-blot and RT-PCR showed that BAFF and BAFFR were significantly higher than the control group expression in epileptic patients and animal models (P0.05). Conclusion: the expression of BAFF and BAFFR in patients with epilepsy and epilepsy model in rats increased significantly. The present study shows that immune and inflammatory reaction of BAFF and BAFFR in the central nervous system, so we speculate that the rise of BAFF And BAFFR can regulate the process of epilepsy by immuno inflammatory response and play a role in the pathogenesis of epilepsy.

【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R742.1;R-332

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