小鼠局灶性脑缺血再灌注后IL-33及其受体的时程表达

发布时间：2018-03-04 22:13

本文选题：脑缺血再灌注　切入点：大脑中动脉栓塞　出处：《中风与神经疾病杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的检测白细胞介素-33(IL-33)及其膜受体ST2和可溶型受体sST2在小鼠局灶性脑缺血再灌注后不同时程的表达特征。方法利用线栓法闭塞大脑中动脉(MCAO)30 min诱导建立小鼠可逆性局灶性脑缺血再灌注损伤模型,通过半定量RT-PCR检测脑缺血再灌注后6 h、24 h和3 d缺血脑组织中IL-33及其膜受体ST2、凋亡相关蛋白Caspase-8和Caspase-3的mRNA表达水平,并通过免疫组织化学染色观察了IL-33在不同缺血脑区(运动皮质、感觉皮质、海马和纹状体)的时程表达情况;ELISA法检测了小鼠MCAO模型再灌注后不同时间点血清中IL-33及其可溶型受体sST2的表达水平。结果 IL-33 mRNA在缺血后6 h和3 d表达减少,但在24 h无明显改变;凋亡相关蛋白Caspase-3和Caspase-8在缺血后3个时间点均显著增高,且Caspase-3在6 h和3 d的mRNA表达水平较24 h高;ST2 mRNA在缺血后6 h无减少,但在24 h和3 d有明显减少;除了MCAO 24 h组运动皮质和纹状体阳性染色增加外,IL-33阳性细胞数在缺血后不同时程各脑区均有不同程度减少;缺血后外周血中IL-33的表达量无明显升高或降低,而sST2的表达水平在缺血后6 h即已显著升高。结论脑缺血再灌注后IL-33/ST2信号通路被下调,其与sST2表达增多的效应发挥和神经元凋亡有关。
[Abstract]:Objective to investigate the expression characteristics of interleukin-33 (IL-33), its membrane receptor ST2 and soluble receptor sST2 after focal cerebral ischemia reperfusion in mice. Methods Induction of 30 min occlusion of middle cerebral artery (MCAO) with thread occlusion was used to establish a reversible model in mice. Model of focal cerebral ischemia-reperfusion injury, The expression of IL-33 and its membrane receptor ST2, apoptosis-related protein (Caspase-8) and Caspase-3 were measured by semi-quantitative RT-PCR at 24 h and 3 d after cerebral ischemia-reperfusion. IL-33 was observed in different ischemic brain regions (motor cortex, sensory cortex) by immunohistochemical staining. The expression of IL-33 and its soluble receptor sST2 in serum at different time points after reperfusion in MCAO model of mice was detected by Elisa. Results the expression of IL-33 mRNA decreased at 6 h and 3 d after ischemia. The expression of apoptosis-related protein Caspase-3 and Caspase-8 increased significantly at 3 hours after ischemia, and the mRNA expression level of Caspase-3 at 6 h and 3 d was higher than that at 24 h after ischemia, but it was significantly decreased at 24 h and 3 d after ischemia. With the exception of MCAO 24 h group, the positive staining of motor cortex and striatum increased the number of IL-33 positive cells in different brain regions after ischemia, but the expression of IL-33 in peripheral blood did not increase or decrease after ischemia. The expression of sST2 increased significantly 6 hours after ischemia. Conclusion the signal pathway of IL-33/ST2 is down-regulated after cerebral ischemia-reperfusion, which is related to the effect of increased sST2 expression and neuronal apoptosis.
【作者单位】：华中科技大学同济医学院附属武汉中心医院风湿与肾内科;武汉大学中南医院检验科;
【基金】：国家自然科学基金(No.81501033)
【分类号】：R743

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