PACAP、BDNF在头痛患者外周血单个核细胞中mRNA表达

发布时间：2018-03-10 17:34

本文选题：头痛　切入点：PACAP　出处：《中国人民解放军医学院》2015年博士论文　论文类型：学位论文

【摘要】：背景头痛在困扰人类的疼痛中是发病频率最高的,也是临床最常见的症状之一。原发性头痛以偏头痛、紧张型头痛、丛集性头痛为多见,继发性头痛中药物过量性头痛是一类特殊的、常见的头痛类型。目前尚无简单可行、准确度高的生物学指标来明确各种头痛的类型。本课题拟通过对头痛患者和健康人群外周血单个核细胞中PACAP、BDNF的表达进行检测,探讨PACAP、BDNF与头痛人群的相关性,为临床头痛诊断及鉴别诊断寻找简便可行、准确度高的生物学指标,为头痛的机制研究提供新的理论依据。全文共包括两部分内容：第一部分PACAP在头痛患者外周血单个核细胞中mRNA表达目的研究头痛人群外周血单个核细胞中PACAP mRNA表达水平方法选择2014年04月～2015年02月间解放军总医院头痛门诊的头痛患者101例,根据I CHD-3 beta版诊断标准分为4组：偏头痛组、紧张型头痛组、丛集性头痛组及药物过量性头痛组;另选健康人35例作为正常对照组。分离外周血单个核细胞,提取总RNA,逆转录合成cDNA,采用Real-time PCR检测,以得到的PACAP基因Ct值与GAPDH基因Ct值的差值作为外周血单个核细胞中PACAP mRNA相对表达含量,对头痛组各组与正常对照组进行比较。结果偏头痛组与正常对照组比较外周血单个核细胞中的PACAP mRNA表达水平降低,差异有统计学意义(P0.05);正常对照组与紧张型头痛组、丛集性头痛组、药物过量性头痛组比较外周血单个核细胞中的PACAP mRNA表达水平无差异表达。结论外周血单个核细胞中的PACAP mRNA表达水平与偏头痛密切相关,与紧张型头痛、丛集性头痛、药物过量性头痛无明显相关。有助于偏头痛与紧张型头痛、丛集性头痛和药物过量性头痛这几种常见头痛类型鉴别,并为以后的机制研究提供新的理论依据。第二部分BDNF在头痛患者外周血单个核细胞中mRNA表达目的研究头痛人群外周血单个核细胞中BDNF mRNA表达水平方法选择2014年04月～2015年02月间解放军总医院头痛门诊头痛患者101例,根据ICHD-3 beta版诊断标准分为4组：偏头痛组、紧张型头痛组、丛集性头痛组及药物过量性头痛组;另选健康人35例作为正常对照组。分离外周血单个核细胞,提取总RNA,逆转录合成cDNA,采用Real-time PCR检测,以得到的BDNF基因Ct值与GAPDH基因Ct值的差值作为外周血单个核细胞中BDNF mRNA相对表达含量,对头痛组各组与正常对照组进行比较。结果正常对照组与偏头痛组、紧张型头痛组、丛集性头痛组、药物过量性头痛组比较外周血单个核细胞中的BDNF mRNA表达水平无差异表达。结论外周血单个核细胞中的BDNF mRNA表达水平与偏头痛、紧张型头痛、丛集性头痛、药物过量性头痛无明显相关。
[Abstract]:Background headache is one of the most frequent and common symptoms of pain in humans. Migraine, tension headache, cluster headache are common in primary headache. Drug overdose headache is a special and common type of headache in secondary headache. The aim of this study was to detect the expression of PACAPnBDNF in peripheral blood mononuclear cells (PBMC) of patients with headache and healthy people to explore the correlation between PACAPnBDNF and headache. To find a simple, feasible and accurate biological index for clinical diagnosis and differential diagnosis of headache. To provide a new theoretical basis for the study of the mechanism of headache. The thesis consists of two parts: part one: mRNA expression of PACAP in peripheral blood mononuclear cells of headache patients objective to study the PACAP mRNA table in peripheral blood mononuclear cells of headache patients. Methods 101 headache patients were selected from the headache clinic of PLA General Hospital from April 2014 to February 2015. According to the diagnostic criteria of I CHD-3 beta, they were divided into four groups: migraine group, tension headache group, cluster headache group and drug overdose headache group; 35 healthy persons were selected as normal control group. Peripheral blood mononuclear cells (PBMC) were isolated. Total RNAs were extracted, reverse transcription was used to synthesize cDNAs, and Real-time PCR was used to detect the difference between Ct value of PACAP gene and Ct value of GAPDH gene as the relative expression content of PACAP mRNA in peripheral blood mononuclear cells. Results the expression of PACAP mRNA in peripheral blood mononuclear cells in migraine group was significantly lower than that in normal control group (P 0.05). There was no difference in the expression of PACAP mRNA in peripheral blood mononuclear cells between cluster headache group and drug overdose headache group. Conclusion the expression of PACAP mRNA in peripheral blood mononuclear cells is closely related to migraine and is associated with tension headache. There is no obvious correlation between cluster headache and drug overdose headache. It is helpful to distinguish migraine from tension headache, cluster headache and drug overdose headache. Part 2: mRNA expression in peripheral blood mononuclear cells of headache patients objective to study the expression level of BDNF mRNA in peripheral blood mononuclear cells of headache patients on 2014. From April to February 2015, there were 101 headache patients in the headache clinic of PLA General Hospital. According to the ICHD-3 beta criteria, the patients were divided into four groups: migraine group, tension headache group, cluster headache group and drug overdose headache group. Total RNAs were extracted, reverse transcription was used to synthesize cDNAs, and Real-time PCR was used to detect the difference between Ct value of BDNF gene and Ct value of GAPDH gene as the relative expression content of BDNF mRNA in peripheral blood mononuclear cells. Results normal control group and migraine group, tension type headache group, cluster headache group, group of cluster headache, There was no difference in the expression of BDNF mRNA in peripheral blood mononuclear cells in the drug overdose headache group. Conclusion the expression of BDNF mRNA in peripheral blood mononuclear cells is similar to migraine, tension headache and cluster headache. There was no significant correlation between drug overdose headache.
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2015
【分类号】：R741

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