TLR4基因多态性与大动脉粥样硬化型脑梗死的相关性研究

发布时间：2018-03-11 00:29

本文选题：TLR4基因　切入点：大动脉粥样硬化型脑梗死　出处：《青岛大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨中国青岛地区汉族人群中TLR4基因rs1927911、rs1927914及rs2149356三个单核苷酸多态性位点(single nucleotide polymorphism,SNP)与大动脉粥样硬化型(large-artery atherosclerosis,LAA)脑梗死的相关性。方法:研究对象为在青岛大学医学院第二附属医院神经内科就诊的缺血性脑梗死患者和同期在我院体检中心查体健康者。按照TOAST分型选出105例LAA型脑梗死患者作为LAA组,以及128例查体健康者作为对照组。所有受试者入院后第二天清晨空腹抽取前臂静脉血3ml,用于相关生化指标的检测及白细胞DNA的提取。采集所有受试者的性别、年龄、吸烟史、饮酒史、高血压病史、糖尿病病史及冠心病病史等基本信息。收集并记录数据。用聚合酶链式反应-限制性片段长度多态性(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,PCR-RFLP)检测TLR4基因中rs1927911和rs1927914两个位点的多态性,用直接测序法检测TLR4基因中rs2149356位点的多态性。应用SPSS17.0软件进行统计学分析。两组间计量资料比较采用t检验,符合正态分布的计量资料用均数±标准差(?)表示;计数资料应用X~2检验;各影响因素与脑梗死相关性采用多元Logistic回归分析,p0.05差异有统计学意义;用Hardy-Weinberg平衡检验样本群体代表性,p0.05具有群体代表性。结果:rs1927911位点基因型频率在LAA组与对照组间的差异有统计学意义(p=0.041),rs1927911位点等位基因频率在LAA组与对照组间的差异没有统计学意义(p=0.754),rs2149356位点基因型频率及等位基因频率在LAA组与对照组间的差异有统计学意义(p=0.002,p=0.006),rs1927914位点基因型频率及等位基因频率在两组间的差异无统计学意义(p=0.472,p=0.956)。结论:TLR4基因rs1927911与rs2149356位点多态性与中国青岛地区汉族人群大动脉粥样硬化型脑梗死具有相关性,而rs1927914位点多态性与LAA型脑梗死未发现有相关性。rs2149356位点的等位基因A可能是LAA型脑梗死的易感基因。
[Abstract]:Objective: To investigate the TLR4 gene in Qingdao Han population China rs1927911, rs1927914 and rs2149356 three single nucleotide polymorphisms (single nucleotide, polymorphism, SNP) and large artery atherosclerosis (large-artery atherosclerosis, LAA) cerebral infarction correlation. Methods: the research object in the neurology clinic of the Second Affiliated Hospital of Qiingdao University School of medicine in ischemic cerebral infarction patients and the same period in our hospital health examination center. According to the classification of TOAST type LAA in 105 cases of patients with cerebral infarction were selected as LAA group, and 128 healthy subjects as control group. All subjects second days after admission, fasting forearm venous blood 3ml, for detecting the biochemical indexes of WBC and DNA collect all subjects of sex, age, smoking history, drinking history, history of hypertension, diabetes and coronary heart disease history. Information. Collect and record data. Using polymerase chain reaction restriction fragment length polymorphism (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, PCR-RFLP) to detect the polymorphism of rs1927911 and rs1927914 of TLR4 gene in two loci, the polymorphism of TLR4 gene was detected in rs2149356 locus by direct sequencing. SPSS17.0 software was used for statistical analysis. Two measurement data between groups were compared by t test, with normal distribution measurement data with standard deviation (?); count data using X~2 test; correlation between influence factors and cerebral infarction by using multivariate Logistic regression analysis, there were significant differences in P0.05; with Hardy-Weinberg balance test sample representative group, P0.05 group has representative. Results: there were statistically significant differences of genotype rs1927911 in LAA between the two groups (p= 0.041), rs1927911 The differences in allele frequency between LAA group and control group had no statistical significance (p=0.754), there was a significant difference in rs2149356 genotype and allele frequencies in the LAA group and the control groups (p=0.002, p=0.006), there were no significant differences in rs1927914 genotype and allele frequencies in the between the two groups (p=0.472, p=0.956). Conclusion: there is correlation between type TLR4 atherosclerosis gene rs1927911 and rs2149356 polymorphism and cerebral infarction in Chinese Han population in Qingdao Chinese, and rs1927914 polymorphism and cerebral infarction in type LAA was found between.Rs2149356 allele A may be a susceptible gene of type LAA of cerebral infarction.

【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743.33

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