神经干细胞移植帕金森病模型大鼠行为学及对炎症因子的调节效应

发布时间：2018-03-13 02:28

本文选题：神经干细胞　切入点：帕金森病　出处：《中国组织工程研究》2017年33期 　论文类型：期刊论文

【摘要】：背景:研究证实,干细胞移植可抑制帕金森病模型动物多巴胺能神经元的丧失,改善其行为学症状,具有一定的治疗作用。目的:观察神经干细胞移植后帕金森病大鼠行为学及炎症因子的变化。方法:将108只SD大鼠随机分为正常对照组、模型组及干细胞移植组,每组36只。模型组及干细胞移植组在脑内纹状体内注射6-羟基多巴胺建立帕金森病模型,造模成功后即刻,干细胞移植组于纹状体内注射1×109 L-1的神经干细胞悬液5μL,模型组注射等量的生理盐水。细胞移植后1,2,4周,3组每个时间点各取大鼠6只,腹腔注射0.5 mg/kg的阿扑吗啡,注射后10 min开始计数旋转圈数,计时5 min;ELISA法检测脑组织内肿瘤坏死因子α、白细胞介素1β、干扰素γ及白细胞介素4水平。结果与结论:(1)正常对照组未发生旋转行为。干细胞移植组与模型组出现旋转行为,干细胞移植组于移植后2周开始旋转次数明显减少,干细胞移植组移植后2,4周的旋转次数明显低于模型组(P0.05),且组内移植后2,4周的旋转次数明显低于移植后1周(P0.05);(2)与正常对照组比较,模型组移植后不同时间点的肿瘤坏死因子α、白细胞介素1β水平明显升高(P0.05);与模型组比较,干细胞移植组移植后不同时间点的肿瘤坏死因子α、白细胞介素1β水平明显降低(P0.05);(3)与正常对照组比较,模型组移植后不同时间点的干扰素γ、白细胞介素4水平均明显升高(P0.05);与模型组比较,干细胞移植组移植后1,4周的干扰素γ、白细胞介素4水平均明显升高(P0.05);(4)结果表明,神经干细胞移植可改善帕金森病大鼠的行为学症状,降低其脑内促炎性因子肿瘤坏死因子α、白细胞介素1β水平,提高抗炎因子白细胞介素4的水平。
[Abstract]:Background: stem cell transplantation has been shown to inhibit the loss of dopaminergic neurons and improve behavioral symptoms in Parkinson's disease model animals. Objective: to observe the changes of behavioral and inflammatory factors in rats with Parkinson's disease after transplantation of neural stem cells. Methods: 108 SD rats were randomly divided into normal control group, model group and stem cell transplantation group. The model group and stem cell transplantation group were injected with 6-hydroxydopamine into the striatum to establish Parkinson's disease model. The stem cell transplantation group was injected with 1 脳 109 L-1 neural stem cell suspension (5 渭 L) in striatum and the model group was injected with the same amount of normal saline. Six rats were taken from each time point in each time point of 1: 2 and 4 weeks after cell transplantation, and the rats were injected intraperitoneally with apomorphine for 0.5 mg/kg. 10 min after injection, the number of rotating circles was counted, The levels of tumor necrosis factor 伪, interleukin 1 尾, interferon 纬 and interleukin 4 in brain tissue were measured by 5 mins Elisa. Results and conclusion: there was no rotational behavior in normal control group, but rotation behavior was observed in stem cell transplantation group and model group. The number of rotation in stem cell transplantation group decreased significantly 2 weeks after transplantation. The rotation times of the stem cell transplantation group at 2 ~ 4 weeks after transplantation were significantly lower than those of the model group (P 0.05), and the rotation times of the group 2 ~ 4 weeks after transplantation were significantly lower than those of the normal control group 1 week after transplantation. The levels of tumor necrosis factor 伪 and interleukin 1 尾 in the model group were significantly higher than those in the model group at different time points (P 0.05). The levels of tumor necrosis factor 伪 and interleukin 1 尾 in stem cell transplantation group were significantly lower than those in normal control group at different time points after transplantation. The levels of interferon 纬 and interleukin 4 at different time points in model group were significantly higher than those in model group, and the levels of interferon 纬 and interleukin 4 in stem cell transplantation group were significantly higher than those in model group at 1 and 4 weeks after transplantation. Neural stem cell transplantation can improve the behavioral symptoms of Parkinson's disease rats, reduce the levels of tumor necrosis factor 伪 and interleukin-1 尾 in the brain and increase the level of anti-inflammatory factor interleukin 4 in the brain of rats with Parkinson's disease.
【作者单位】：湖北职业技术学院医学院;四川省人民医院;
【基金】：四川省卫生厅科研课题(110238)~~
【分类号】：R-332;R742.5

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