病灶区灌注罗格列酮对脑出血家兔血肿周围咬合蛋白和闭合小环蛋白1 mRNA表达水平及血-脑屏障通透性的影响

发布时间：2018-03-15 09:48

本文选题：脑出血　切入点：罗格列酮　出处：《中国脑血管病杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的观察病灶区灌注罗格列酮(RSG)对家兔脑出血(ICH)模型血肿周围咬合蛋白和闭合小环蛋白1(ZO-1)mRNA表达水平、血-脑屏障(BBB)通透性及神经功能评分的影响。方法选择健康雄性家兔45只,体质量2.0~2.5 kg,按随机数字表法分为对照组、ICH模型组和罗格列酮(RSG)治疗组,每组15只(其中5只用于BBB测定)。对照组模拟制作颅内血肿的过程,穿刺成功后对兔靶点注射等渗盐水0.3 ml,6 h后再次注入等渗盐水0.1 ml;ICH模型组穿刺成功后靶点注射自体非抗凝动脉血0.3 ml,6 h后靶点再次注入等渗盐水0.1 ml;RSG治疗组在ICH模型制作成功后6 h,血肿区灌注RSG 0.5 mg(溶于0.1 ml等渗盐水中)。继续饲养至第7天,对各组家兔进行神经功能缺损评分(Purdy评分)后处死,逆转录聚合酶链反应(RT-PCR)法检测血肿周围脑组织咬合蛋白及ZO-1 mRNA的表达水平;采用甲酰胺方法测量血肿周围脑组织伊文思蓝(EB)含量以评估BBB通透性。结果 (1)神经功能评分:对照组、ICH模型组、RSG治疗组Purdy评分分别为(2.53±0.05)、(8.13±0.06)、(6.67±0.08)分,组间差异有统计学意义(F=459.116,P0.01);与对照组比较,ICH模型组、RSG治疗组的Purdy评分均明显增加(均P0.01);与ICH模型组比较,RSG治疗组的Purdy评分降低(P0.05);(2)咬合蛋白及ZO-1 mRNA表达水平:对照组、ICH模型组、RSG治疗组咬合蛋白及ZO-1 mRNA差异均有统计学意义(每组分别为1.013±0.051、1.001±0.045;0.221±0.017、0.247±0.019;0.498±0.041、0.613±0.045;F值分别为443.924、381.929,均P0.01),与对照组比较,ICH模型组、RSG治疗组咬合蛋白及ZO-1 mRNA表达均显著减少(均P0.01);与ICH模型组比较,RSG治疗组咬合蛋白及ZO-1 mRNA的表达增加(均P0.05);(3)BBB通透性:对照组、ICH模型组、RSG治疗组EB含量分别为(12.0±1.0)、(51.6±0.9)、(36.4±1.0)μg/g,组间差异有统计学意义(F=223.516,P0.01);与对照组比较,ICH模型组、RSG治疗组的EB含量明显增加(均P0.01);与ICH模型组比较,RSG治疗组EB含量明显降低(P0.01)。结论病灶区灌注RSG可明显改善家兔ICH后的神经功能,增加血肿周围脑组织咬合蛋白和ZO-1mRNA的表达水平,降低BBB通透性。
[Abstract]:Objective to observe the effects of rosiglitazone RSGs on the expression of occlusal protein and closed microannular protein 1a ZO-1mRNA, BBB permeability and neurological function score of intracerebral hemorrhage (ICH) model in rabbits. Methods 45 healthy male rabbits were selected. The rats were randomly divided into two groups: control group (n = 15) and rosiglitazone group (n = 15). The control group was used to simulate the process of making intracranial hematoma. After successful puncture, rabbit target was injected with isoosmotic saline 0.3 ml / h and then re-injected 0.1 ml / ml ICH after puncture. In the model group, the target was injected with autogenous non-anticoagulant arterial blood 0.3 ml / min 6 h later, then the target was re-injected with 0.1 ml / L isoosmotic saline in ICH group. 6 hours after the model was successfully made, RSG 0.5 mg (dissolved in 0.1 ml isoosmotic saline) was infused into the hematoma area. The rabbits in each group were killed after the neurological impairment score (Purdy score). The expression of occlusal protein and ZO-1 mRNA were detected by reverse transcriptase polymerase chain reaction (RT-PCR) in the brain tissue around the hematoma. The BBB permeability was evaluated by formamide method. Results: the Purdy score of the control group was 2.53 卤0.05 卤0.05, and the Purdy score was 6.67 卤0.08 in the control group. There was significant difference between the two groups (P < 0.01); compared with the control group, the Purdy score of the treated group increased significantly (all P 0.01; compared with the ICH model group, the Purdy score of the Purdy group decreased the level of P0.05P0.05) and the expression of ZO-1 mRNA in the control group was significantly higher than that in the control group. The occlusal protein and ZO-1 mRNA in the ICH model group were significantly lower than those in the control group (1.013 卤0.051 卤1.001 卤0.045 卤0.221 卤0.017) 0.247 卤0.041 卤0.498 卤0.041 卤0.613 卤0.045 F, respectively (P 0.01). Compared with the control group, the occlusal protein and ZO-1 mRNA expression in the ICH model group were significantly decreased (P 0.01). The expression of occlusal protein and ZO-1 mRNA in the ICH model group were significantly higher than those in the control group (P0.05). The EB content in the control group was 12.0 卤1.0 卤1.0 卤0.96 卤0.96 卤0.96 卤1.0 渭 g / g, respectively. The difference between the two groups was statistically significant (P < 0.05), and that of the control group was higher than that of the control group (P < 0.05). Compared with the ICH model group, the EB content in the treatment group was significantly lower than that in the control group. Conclusion the perfusion of RSG in the focus area can obviously improve the nerve function after ICH in rabbits. The expression of occlusal protein and ZO-1mRNA were increased and the permeability of BBB was decreased.
【作者单位】：贵州医科大学附属医院急诊医学科;
【基金】：国家自然科学基金(81460185/H09106) 贵阳市科技计划项目(20161001-51)
【分类号】：R743.34

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