应用脑脊液蛋白芯片检测技术对结核性脑膜炎诊断价值的研究

发布时间：2018-03-17 06:37

本文选题：结核性脑膜炎　切入点：脑脊液　出处：《宁夏医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的探讨结核分枝杆菌蛋白芯片检测脑脊液中结核抗体诊断结核性脑膜炎的应用价值。方法应用结核蛋白芯片检测30例临床诊断结核性脑膜炎患者脑脊液及血清标本，30例非结核性脑膜炎患者脑脊液标本，并对实验结果进行分析评价。结果结核芯片法检测脑脊液敏感性、特异性、阳性预测值、阴性预测值、约登指数分别为63.33%、96.67%、95.00%、72.50%、60.00%，结核芯片法检测血清敏感性为66.67%。比较结脑组脑脊液及血清阳性率，差异无统计学意义。（2=0.073，p=0.787，p0.05）。结论结核蛋白芯片是一种集基因重组技术、斑点免疫渗滤技术与微阵列技术于一身的微量分析技术，应用结核蛋白芯片检测脑脊液中特异性结核抗体可能成为诊断结核性脑膜炎的重要辅助检查方法。
[Abstract]:Objective to evaluate the value of detecting tuberculosis antibody in cerebrospinal fluid (CSF) by protein chip of Mycobacterium tuberculosis in diagnosis of tuberculous meningitis. Methods Cerebrospinal fluid (CSF) and serum samples from 30 patients with tuberculous meningitis were detected by tuberculous protein chip. The results were analyzed and evaluated in 30 patients with non-tuberculous meningitis. Results the sensitivity, specificity, positive predictive value, negative predictive value and Yorden index of tuberculous microarray were 63.33, 96.67 and 72.50%, respectively. The sensitivity of tuberculosis microarray was 66.67. The positive rates of CSF and serum were compared. The difference was not statistically significant. Conclusion Tuberculosis protein microarray is a microanalysis technique which integrates gene recombination technique, dot immunofiltration technique and microarray technique. The detection of specific tuberculosis antibody in cerebrospinal fluid with TB protein chip may be an important auxiliary examination method for diagnosis of tuberculous meningitis.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R529.3

【参考文献】