癫痫持续状态大鼠海马Bim与Caspase-3的表达

发布时间：2018-03-19 21:05

本文选题：癫痫持续状态　切入点：Bcl-相互作用细胞死亡介导因子(Bim)　出处：《神经解剖学杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的:探讨在匹罗卡品致痫的癫痫持续状态(Status epilepticus,SE)大鼠模型中,Bcl-2-相互作用细胞死亡介导因子(Bim)与Caspase-3在大鼠海马组织中的表达。方法:雄性成年Sprague-Dawley(SD)大鼠,应用匹罗卡品产生SE 60 min后终止发作,24 h后取材,行HE染色及TUNEL染色,观察海马神经元的损伤及凋亡情况,Western Blot测定海马组织中Bim的表达,免疫组化检测Caspase-3的表达。采用Pearson相关分析法分析Bim与Caspase-3表达的相关性。结果:SE后24 h,海马组织HE染色见神经元数量减少,TUNEL染色阳性细胞数明显增加,Western Blot中Bim出现相对分子量26kD的条带,免疫组化染色可见大量Caspase-3阳性神经元。结论:大鼠SE后24 h,Bim与Caspase-3在海马组织神经元表达增加,且二者的表达呈正相关(r=0.901,P=0.001),海马神经元出现凋亡。
[Abstract]:Objective: to investigate the expression of Bcl-2- and Caspase-3 in hippocampal tissue of rats with epileptic status epilepticus induced by pilocarpine. Methods: adult Sprague-Dawley SD rats were used as control group. Using pilocarpine to produce SE 60 min and stop attack 24 h later, samples were collected, HE staining and TUNEL staining were used to observe the injury and apoptosis of hippocampal neurons. Western Blot was used to detect the expression of Bim in hippocampal tissue. Immunohistochemistry was used to detect the expression of Caspase-3. Pearson correlation analysis was used to analyze the correlation between Bim and Caspase-3 expression. Results the number of neurons decreased and the number of Tunel positive cells increased significantly in hippocampal tissue 24 h after 0: SE. Bim appeared in Western Blot. Relative molecular weight of 26kD band, Conclusion: the expression of Bim and Caspase-3 in hippocampal neurons increased 24 h after SE, and there was a positive correlation between the expression of BIM and Caspase-3 in hippocampal neurons and apoptosis of hippocampal neurons.
【作者单位】：苏州大学医学部;上海交通大学附属第六人民医院神经内科;
【分类号】：R742.1

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