小胶质细胞对中脑多巴胺能神经元铁代谢的影响及其机制:体外细胞学实验方案（英文）

发布时间：2018-03-20 09:24

本文选题：小神经胶质细胞　切入点：帕金森病　出处：《中国组织工程研究》2017年08期 　论文类型：期刊论文

【摘要】：背景:目前,铁代谢障碍已被公认为帕金森发病的一个关键因素,但是脑铁代谢的研究仍处于起步阶段,胶质细胞在脑铁代谢中所扮演的角色还不清楚。许多研究显示,小胶质细胞在帕金森发病中起重要的作用,且与铁的关系密切。目的:在前期研究的基础上,深入探讨小胶质细胞与神经元在铁代谢领域的关系,阐明小胶质细胞在铁选择性损伤多巴胺能神经元致帕金森中的作用。方法:实验为体外细胞学实验,在中国山东,青岛大学医学院完成。(1)改变小胶质细胞铁的水平,观察小胶质细胞条件培养基对中脑神经元的存活率及铁代谢的影响。用脂多糖激活不同铁水平的小胶质细胞,观察小胶质细胞条件培养基对中脑神经元存活率及铁代谢的影响;(2)应用小干涉RNA、ELASA方法观察小胶质细胞内不同的铁水平对小胶质细胞及其激活时所释放的炎症因子和乳铁蛋白的影响,观察小胶质细胞释放的炎症因子和乳铁蛋白对神经元存活率及铁代谢的影响;(3)改变中脑神经元的铁水平,然后加入上述小胶质细胞条件培养基,观察中脑神经元存活率及铁代谢的变化。实验方案经医院伦理委员会批准,批准号为01482 311234。大鼠的实验操作和取材遵循《关于善待实验动物的指导性意见》规定,并与美国国立卫生与健康研究院的指南一致。结果与结论:实验证实了小胶质细胞的功能与黑质多巴胺能神经元铁代谢密切相关,如何调控小胶质细胞的功能使其向保护神经元的方向发展,这一方面的研究进展无疑将对帕金森病的防治有极大的推动作用。
[Abstract]:Background: at present, iron metabolism disorder has been recognized as a key factor in Parkinson's disease, but the study of brain iron metabolism is still in its infancy, and the role of glial cells in brain iron metabolism is not clear. Microglia play an important role in the pathogenesis of Parkinson's disease and are closely related to iron. Objective: to explore the relationship between microglia and neurons in iron metabolism on the basis of previous studies. To elucidate the role of microglia in Parkinson's disease induced by iron selective injury of dopaminergic neurons. Methods: the experiment was carried out in vitro cytology, completed at the School of Medicine, Qingdao University, Shandong, China) and changed the level of iron in microglia cells. To observe the effect of microglial conditioned medium on the survival rate and iron metabolism of mesencephalic neurons. Lipopolysaccharide was used to activate microglia with different iron levels. Effects of microglial conditioned medium on the survival rate and iron metabolism of mesencephalic neurons. (2) small interference RNA-ELASA method was used to observe the effect of different iron levels in microglia on microglial cells and the inflammatory factors released during activation. And lactoferrin, To observe the effect of inflammatory factors released by microglia and lactoferrin on the survival rate and iron metabolism of neurons. To observe the changes of survival rate and iron metabolism of mesencephalic neurons. The experimental plan was approved by the Hospital Ethics Committee and approved as 01482 311234. The experimental operation and material acquisition of the rats were in accordance with the guidelines on treating the Experimental Animals well. Results and conclusions: the function of microglia is closely related to the iron metabolism of dopaminergic neurons in the substantia nigra. How to regulate the function of microglia to protect neurons will undoubtedly play an important role in the prevention and treatment of Parkinson's disease.
【作者单位】：青岛大学医学院;
【基金】：the National Natural Science Foundation of China,No.99020288~~
【分类号】：R742.5

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