脑膜瘤中5-LOX的表达及其意义

发布时间：2018-03-22 09:43

  本文选题：5-脂氧合酶　切入点：脑膜瘤　出处：《泸州医学院》2014年硕士论文　论文类型：学位论文

【摘要】：目的:通过检测5-脂氧合酶（5-lipoxygenase，5-LOX）在脑膜瘤组织中的表达情况，以探讨其在脑膜瘤的发生和发展过程中的意义。方法:①实验用标本来自于2012年3月至2013年1月泸州医学院附属医院神经外科通过开颅手术切除且由病理检查确诊的脑膜瘤组织，共65例。全部病例在本次手术前都没进行过规范的化疗和（或）放射治疗或其它相关治疗，且各脑膜瘤标本的病理资料和临床资料（包括影像资料）均完整。对照组为正常脑膜组织（均为蛛网膜组织），均来自于急性重型闭合性颅脑外伤行内外减压术的患者，共15例。②将脑膜瘤标本制备后按世界卫生组织（WHO）2007年标准对其进行病理分级并分为两组。③所有的对照组标本均在分离人体后12小时之内进行冷冻、固定处理。④对脑膜瘤及正常脑膜组织进行免疫组化链霉菌抗生物素蛋白-过氧化物酶连结（S-P）法染色处理，检测其中的5-LOX表达情况。⑤由两名统计人员进行观察和统计5-LOX在脑膜瘤组织中和蛛网膜中的表达情况（主要是5-LOX在细胞中阳性表达的位置和颜色以及阳性表达细胞在所选视野中的比例等）。结果:①5-LOX在蛛网膜组织中表达11例阴性，4例弱阳性；在脑膜瘤组织中的表达8例阴性，20例弱阳性，19例阳性，18例强阳性，两者之间差异有显著性（2=15.886，，P=0.0000.05)。②5-LOX在Ⅰ级脑膜瘤组织表达中,8例阴性，19例弱阳性，15例阳性及14例强阳性；在Ⅱ级脑膜瘤组织表达中，1例弱阳性，4例阳性和4例强阳性，无阴性表达例数。二者之间差异有显著性（2=4.353，p=0.0380.05）。③Ⅰ级脑膜瘤伴有瘤周水肿的有23例，水肿率41.2%，Ⅱ级脑膜瘤伴有瘤周水肿的有8例，水肿率88.9%，差异有显著性（2=7.107，p=0.0080.05）。④在Ⅰ级脑膜瘤中，5-LOX的阳性表达率为44.1%（阳性例数和强阳性例数之和占Ⅰ级脑膜瘤标本总数的比例），与瘤周水肿呈正相关关系（2=5.731，p 0.05，=0.304）。在Ⅱ级脑膜瘤中，5-LOX的阳性表达率为88.9%（阳性例数和强阳性例数之和占Ⅱ级脑膜瘤标本总数的比例），与瘤周水肿呈正相关关系（2=9，p0.05，=0.707）。⑤27例男性患者中，24例为Ⅰ级，3例为Ⅱ级；38例女性患者中，32例为Ⅰ级，6例为Ⅱ级，性别与肿瘤级别之间差异无显者性（2=0.29，p=0.590.05）。⑥27例男性患者中，11例5-LOX表达呈阴性，16例5-LOX表达呈阳性；38例女性患者中，17例5-LOX表达呈阴性，21例5-LOX表达呈阳性，性别与肿瘤中5-LOX的表达之间差异无显著性（2=0.103，p=0.7490.05）。结论:①5-LOX在脑膜瘤组织中呈过度表达，推测5-LOX的过度表达可能参与了脑膜瘤的发生、发展。②5-LOX在Ⅰ、Ⅱ级脑膜瘤组织中的表达差异有显著性，可以推测5-LOX的过度表达可能与脑膜瘤的分级之间存在联系。③5-LOX在Ⅰ级脑膜瘤组织中的阳性表达率低于在Ⅱ级脑膜瘤组织中的表达率，而Ⅰ级脑膜瘤瘤周水肿率也低于Ⅱ级脑膜瘤瘤周水肿率，说明5-LOX的阳性表达与瘤周水肿之间可能存在一定的关联性。④性别与脑膜瘤的级别以及肿瘤中5-LOX的表达无明确关系。
[Abstract]:Objective: to detect 5- lipoxygenase (5-lipoxygenase, 5-LOX) expression in meningiomas, and to study the occurrence and development of meningioma in significance. Methods: the experimental samples were collected from March 2012 to January 2013, the Department of Neurosurgery of Affiliated Hospital of Luzhou Medical College by craniotomy and resection of the pathological diagnosis of meningioma, 65 cases. All the cases in the surgery have not been standardized chemotherapy and (or) radiotherapy or other related treatment, and pathological data and clinical data of the meningioma specimens (including image data) are complete. The control group was normal brain tissue (both arachnoid tissue), from acute severe closed craniocerebral trauma underwent decompressive craniotomy in patients with a total of 15 cases. The meningioma specimens were prepared according to the WHO (WHO) 2007 the standard pathological grading and divided into All of the two groups. The control group were in the 12 hours after the separation of body within the fixed freezing processing. Meningiomas and normal brain tissues by immunohistochemical streptavidin Perosidase (S-P) treatment staining method to detect the 5-LOX expression. The expression of the two statistics personnel of observation and statistics of 5-LOX in meningioma tissues and arachnoid in (mainly 5-LOX in cells with positive expression of color and position as well as positive cells in the selected field ratio). Results: the expression of 5-LOX in 11 cases of negative arachnoid tissue, 4 cases weakly positive; 8 cases of negative expression in meningiomas, 20 cases weakly positive, 19 positive cases, 18 cases were strongly positive, there was significant difference between them (2=15.886, P=0.0000.05). The expression of 5-LOX in grade I meningioma, 8 cases were negative, 19 cases weakly positive, 15 cases of Yang 鎬у強14渚嬪己闃虫

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