DTI对脑梗死大鼠下调脑区激活的动态评估

发布时间：2018-03-24 02:41

本文选题：丁苯酞　切入点：DTI　出处：《临床放射学杂志》2017年10期

【摘要】：目的探讨DTI对脑梗死大鼠下调脑区激活的动态评估。方法 120只成年雄性SD大鼠,随机分为假手术组,模型组,生理盐水组,丁苯酞干预组。选取缺血后1 h、3 h、6 h、12 h、24 h,进行神经功能评分,MRI扫描,免疫组织化学观察RGMa蛋白的表达情况。结果 (1)MCAO模型组、生理盐水组大鼠缺血后,不同时间点小脑半球的RGMa蛋白阳性表达均有所增加。丁苯酞组在相应时间点的RGMa蛋白的表达较MCAO模型组有所降低,在1 h、3 h、6 h、12 h差异具有统计学意义。(3)MCAO模型组、生理盐水组大鼠小脑半球缺血后1 h后,各个时间点FA值较假手术组降低,均具有统计学差异(P0.05);丁苯酞组大鼠小脑半球FA值较MCAO模型组升高,在3 h、6 h、12 h、24 h时间点两组间的FA值差异具有统计学意义(P0.05)。(3)各手术组大鼠的m NSS神经功能评分与小脑半球RGMa蛋白表达存在正相关,与FA值呈负相关。结论 DTI可以动态评估脑梗死大鼠下调脑区的激活,为临床预后评估提供影像学依据。
[Abstract]:Objective to investigate the dynamic evaluation of down-regulation of brain activation in rats with cerebral infarction by DTI. Methods 120 adult male SD rats were randomly divided into sham-operation group, model group and saline group. In butyphthalide intervention group, 1 hour after ischemia, 3 hours after ischemia and 6 hours after ischemia, 12 hours and 24 hours after ischemia, the neurological function score was evaluated and the expression of RGMa protein was observed by immunohistochemistry. The expression of RGMa protein in the butyphthalide group was lower than that in the MCAO model group at the corresponding time point, and the difference was statistically significant at 1 h, 3 h, 6 h and 12 h, respectively. The FA value of cerebellar hemisphere in normal saline group was significantly lower than that in sham operation group (P 0.05), and that in butyphthalide group was higher than that in MCAO model group. There was a significant difference in FA value between the two groups at 3 h and 6 h, 12 h and 24 h. There was a positive correlation between the m NSS neurological function score and the expression of RGMa protein in the cerebellar hemisphere of the rats in each group. Conclusion DTI can dynamically evaluate the activation of down-regulated cerebral area in rats with cerebral infarction and provide imaging evidence for clinical prognosis evaluation.
【作者单位】：郑州大学第一附属医院磁共振科;
【基金】：2014年国家自然科学基金项目(编号:U1404823)
【分类号】：R743.3

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