偏头痛大鼠的血浆代谢组学研究

发布时间：2018-03-26 08:14

本文选题：偏头痛　切入点：代谢组学　出处：《中国临床研究》2016年12期

【摘要】：目的运用代谢组学方法,研究大鼠偏头痛的发病机制。方法采用改进的Christina tassorelli方法建立大鼠偏头痛模型(模型组)并设正常对照组。取大鼠血样,通过超高效液相色谱仪结合高分辨率质谱仪联用(UPLCQ-TOF/MS)对模型组与正常对照组血浆进行代谢谱检测。通过Micromass Markerlynx软件对采样数据进行归一化处理。统计学分析使用SIMCA-P+软件偏最小二乘法进行模式识别。结果 UPLC-Q-TOF代谢色谱图显示,与正常对照组相比,模型组的UPLC图出峰量明显增多,而且两组图谱之间有一定程度的关联性。散点分布图显示,正常对照组和模型组血样标本均具有明显的聚类作用,且有趋势显著的分类运动。模型组与正常对照组的荷载图并结合MS-MS的串联质谱结果显示,5-羟吲哚乙酸、3,4-二羟苯乙二醇、磷脂酰胆碱为潜在的生物标志物。结论本研究建立了基于大鼠血样的UPLC-Q-TOF/MS代谢组学研究方法,初步阐释了偏头痛发病的生物标志物及代谢组学机制。
[Abstract]:Objective to study the pathogenesis of migraine in rats by metabolic method. Methods the migraine model (model group) was established by modified Christina tassorelli method and the normal control group was set up. The plasma metabolism spectrum of the model group and the normal control group was detected by the combination of UPLCQ-TOF / MS with ultra-high performance liquid chromatograph and high resolution mass spectrometer. The sample data were normalized by Micromass Markerlynx software. SIMCA-P soft was used for statistical analysis. The partial least square method was used for pattern recognition. Results UPLC-Q-TOF metabolic chromatogram showed that, Compared with the normal control group, the peak amount of UPLC map in the model group was significantly increased, and there was a certain degree of correlation between the two groups. The scattered point map showed that the blood samples of the normal control group and the model group had obvious clustering effect. The load diagram of the model group and the normal control group, combined with the results of MS-MS tandem mass spectrometry, showed that 5-hydroxyindoleacetic acid 3o 4-dihydroxybenzeneglycol, Conclusion in this study, UPLC-Q-TOF/MS metabolomics based on rat blood samples was established, and the biomarkers and metabolic mechanism of migraine were preliminarily explained.
【作者单位】：广州市红十字会医院中医科;南方医科大学中医药学院;
【基金】：国家自然科学基金(81202687) 广东省科技计划项目(2012B061700018) 广东省中医药管理局科研课题(20141218)
【分类号】：R747.2

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