颞叶癫痫模型幼鼠和成年大鼠发作时间、发作类型的差异及发作后Ang-2蛋白表达变化

发布时间：2018-03-26 13:37

  本文选题：癫痫　切入点：动物模型　出处：《广东医学》2017年21期

【摘要】：目的对比观察颞叶癫痫模型幼鼠和成年大鼠在发作时间、发作类型的差异,分析癫痫发作是否具有年龄依赖性;并观察两组大鼠颞叶癫痫发作后海马区血管内皮系统促血管生成素2(Ang-2)的蛋白表达变化及差异。方法选取健康雄性Wistar大鼠,分为幼年组(2~3周,体重30~60 g)和成年组(7~8周,体重200~250 g),各32只,均随机分为正常对照组(12只)及实验组(20只)。按Racine分级标准观察大鼠行为改变,并记录各组大鼠癫痫发作时间及情况。正常对照组腹腔注射生理盐水。正常对照组大鼠癫痫持续状态后第1、3、5、7天颈椎脱臼处死,断头取海马组织,采用Western blot技术观察Ang-2的蛋白表达变化。结果正常对照组大鼠均未发生任何癫痫样发作表现;实验组大鼠从给药至出现癫痫样发作Ⅳ级时间,幼鼠为(12.7±5.12)min,成年鼠为(25.3±6.71)min;幼鼠癫痫发作程度较成年鼠严重;首发癫痫持续状态(SE)达Ⅳ级及Ⅳ级以上者占90%,成年鼠为50%;癫痫发作后10%成年鼠可自行终止,幼鼠不能自行终止,且均发展为癫痫持续状态;幼鼠死亡率10%,成年鼠无死亡。幼鼠及成年大鼠的正常对照组在腹腔注射生理盐水后第1、3、5、7天,海马中Ang-2蛋白表达无明显改变(P0.01);与正常对照组相比,癫痫持续状态后幼鼠及成年大鼠海马组织Ang-2蛋白表达均升高(P0.05),且幼鼠升高程度较成年鼠高(P0.05)。癫痫持续状态后第1天起,幼鼠及成年鼠海马中Ang-2蛋白表达均逐渐升高,第5天达高峰,后下降(P0.05)。结论癫痫发作具有年龄依赖性;Ang-2蛋白表达水平与癫痫发作相关。
[Abstract]:Objective to compare the time and type of temporal lobe epilepsy between young and adult rats, and to analyze whether the seizure is age-dependent. The changes and differences of protein expression of vascular endothelial system angiopoietin-2 (Ang-2) in hippocampal area after temporal lobe epilepsy were observed in two groups. Methods healthy male Wistar rats were divided into juvenile group (3 weeks) and adult group (30 ~ 60 g) and adult group (7 ~ 8 weeks). A total of 32 rats were randomly divided into normal control group (n = 12) and experimental group (n = 20). The behavioral changes of rats were observed according to Racine grading standard. The time and condition of epileptic seizures in each group were recorded. The normal control group was injected with normal saline intraperitoneally. The rats in the normal control group were killed by cervical dislocated and severed hippocampus on the 7th day after epileptic status. Western blot technique was used to observe the expression of Ang-2 protein. Results there was no epileptic seizure in the normal control group, while in the experimental group, the time from the administration of the drug to the onset of epileptiform seizure grade 鈪，

本文编号：1668078