厄贝沙坦对帕金森病大鼠的保护作用

发布时间：2018-03-27 11:36

本文选题：帕金森病　切入点：鱼藤酮　出处：《中国老年学杂志》2017年22期

【摘要】：目的探讨厄贝沙坦对鱼藤酮致帕金森病(PD)大鼠模型的保护作用。方法 48只大鼠按随机数字表法分成对照组、PD模型组、PD+厄贝沙坦组、厄贝沙坦组,每组12只。2.5 mg·kg~(-1)·d~(-1)鱼藤酮溶于二甲基亚砜和聚乙二醇(1∶1)混合液中,埋于PD模型组、PD+厄贝沙坦组微量渗透泵中,余2组给予等质量的生理盐水;PD+厄贝沙坦组、厄贝沙坦组同时给予1 mg·kg~(-1)·d~(-1)厄贝沙坦每天上午8时灌胃,其余2组给予等体积生理盐水灌胃,试验周期为4 w。行为学检测评定运动功能等损害症状;高效液相检测黑质区多巴胺含量;免疫组化染色检测大鼠黑质区酪氨酸羟化酶(TH)阳性细胞数目;Western印迹检测黑质部位α-共核蛋白、caspase-3、核因子(NF)-κB蛋白表达水平。结果与对照组比较,PD模型组体质量、多巴胺含量显著下降,黑质区TH阳性细胞数目明显减少,α-共核蛋白、caspase-3、NF-κB蛋白表达水平显著上升(P0.05);但与PD模型组比较,PD+厄贝沙坦组经厄贝沙坦干预后,体质量、多巴胺含量显著提高,TH阳性细胞数目显著增加,α-共核蛋白、caspase-3、NF-κB蛋白表达显著下调(P0.05)。结论厄贝沙坦可通过提高多巴胺含量、抑制黑质区TH阳性细胞凋亡和下调α-共核蛋白、caspase-3、NF-κB蛋白表达而起到鱼藤酮致PD模型大鼠的保护作用。
[Abstract]:Objective to investigate the protective effect of irbesartan on rat model of Parkinson's disease induced by rotenone. Methods 48 rats were randomly divided into control group (PD model group) and irbesartan group (PD group). Rotenone was dissolved in the mixture of dimethyl sulfoxide (DMSO) and polyethylene glycol (1: 1), buried in PD irbesartan group (PD model group), and in PD Irbesartan group (microosmotic pump). The remaining two groups were treated with normal saline and PD irbesartan of the same quality. Irbesartan group was given 1 mg / kg / d ~ (-1)) irbesartan was perfused by stomach at 8 a.m. and the other two groups were given normal saline of the same volume for 4 ws.Behavioral examination was performed to evaluate motor function and other symptoms. The content of dopamine in substantia nigra was detected by HPLC. The number of tyrosine hydroxylase tyrosine hydroxylase (THH) positive cells in substantia nigra was detected by immunohistochemical staining and Western blot was used to detect the expression of 伪 -eukaryotic protein caspase-3 and NF-魏 B protein in substantia nigra. The content of dopamine decreased significantly, the number of th positive cells in substantia nigra decreased significantly, and the expression of 伪 -eukaryotic protein caspase-3, NF- 魏 B increased significantly (P 0.05), but compared with PD model group, the body weight of PD irbesartan group was increased after irbesartan intervention. Dopamine content significantly increased the number of TH-positive cells, and the expression of 伪 -eukaryotic protein caspase-3 and NF- 魏 B decreased significantly (P 0.05). Conclusion Irbesartan can increase dopamine content. The protective effect of rotenone on PD rats was observed by inhibiting the apoptosis of th positive cells and down-regulating the expression of 伪 -eukaryotic protein caspase-3 and NF- 魏 B in substantia nigra.
【作者单位】：青海大学附属医院老年病科;玉林师范学院生物与制药学院;
【基金】：广西自然科学基金资助项目(No.2016GXNSFBA380040)
【分类号】：R742.5

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