脂多糖LPS诱导的神经炎症对小鼠认知功能的影响及相关机制分析

发布时间：2018-03-30 14:06

本文选题：脂多糖　切入点：小胶质细胞　出处：《基因组学与应用生物学》2017年04期

【摘要】：为研究脂多糖(lipopolysaccharide,LPS)对小鼠认知功能的影响,本研究将18只C57BL/6小鼠随机分为两组,即正常对照组和LPS处理组,每组各9只小鼠。在腹腔注射LPS 24 h后行水迷宫实验检测小鼠行为学变化。此外,本研究采用Western blotting和免疫组化检测小鼠脑内小胶质细胞特异性标记物离子通道相关钙衔接蛋白(ionized calcium binding adapter,IBA1)的表达,TUNEL凋亡法检测小鼠脑内神经元凋亡情况。实验结果发现LPS处理后小鼠认知功能下降。Western blotting和免疫组化结果均提示LPS处理后小鼠脑中IBA1表达量增加,小胶质细胞激活;TUNEL提示LPS处理后小鼠脑内出现大量凋亡神经元,由此推断LPS可能通过激活小胶质细胞,扩大神经炎症反应,增加神经元凋亡导致小鼠认知功能下降。
[Abstract]:In order to study the effect of lipopolysaccharide on cognitive function in mice, 18 C57BL/6 mice were randomly divided into two groups: normal control group and LPS treated group. Nine mice in each group were given intraperitoneal injection of LPS for 24 hours to detect the behavioral changes of mice by water maze test. In this study, Western blotting and immunohistochemistry were used to detect the expression of ionized calcium binding adapterated IBA 1) in the brain of mice. The apoptosis of neurons in the brain was detected by Tunel method. After LPS treatment, the cognitive function of mice decreased. The results of Western blotting and immunohistochemistry showed that the expression of IBA1 in the brain of mice treated with LPS was increased after LPS treatment. Tunel suggested that there were a large number of apoptotic neurons in the brain of mice treated with LPS. It was concluded that LPS may increase neuronal inflammation by activating microglia, and increase neuronal apoptosis, which may lead to the decrease of cognitive function in mice.
【作者单位】：重庆医科大学附属第一医院老年病科神经与心理疾病组;重庆医科大学神经科学中心;
【基金】：重庆市应用开发一般项目(cstc2014yykf A110002) 国家临床重点专科建设资助项目(国卫办医函[2013]544号)共同资助
【分类号】：R741

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