MiR-124在脑血流灌注不足中的表达变化及其作用

发布时间：2018-04-01 00:11

本文选题：miRNA　切入点：脑缺血　出处：《重庆医科大学学报》2017年03期

【摘要】：目的:研究miR-124在脑血流灌注不足大鼠中的动态表达变化,并探讨其在脑血流灌注不足中发挥的作用。方法:采用双侧颈总动脉结扎(Permanent bilateral comman carotid artery occlusion或Two-vessel occlusion,2VO)制备大鼠脑缺血模型,用qRT-PCR的方法在不同的时间点(术后1、7、14、21、28 d)检测miR-124的表达变化。并在SH-SY5Y细胞系中采用流式细胞仪研究低氧时过表达miR-124对细胞周期的影响。结果:与对照组比较,2VO术后大鼠脑皮质血流经历了先下降再上升的动态变化,而miR-124也呈现先下降再上升的动态过程,于术后第7天达到最低(P=0.036);在低氧环境中,过表达miR-124可以将神经细胞系SH-SY5Y的阻止在G1期。结论:miR-124可能参与了大鼠脑血流灌注不足后脑缺血的发生发展,为治疗脑缺血提供了一种新的治疗策略。
[Abstract]:Objective: to study the dynamic expression of miR-124 in rats with cerebral blood perfusion deficiency and its role in cerebral perfusion insufficiency. Methods: bilateral common carotid artery ligation of permanent bilateral comman carotid artery occlusion or Two-vessel occlusion 2VO) was used to establish cerebral ischemia model in rats. QRT-PCR was used to detect the expression of miR-124 at different time points (1: 7, 14, 21, 28 days after operation). Flow cytometry was used to study the effect of overexpression of miR-124 on cell cycle in SH-SY5Y cell line. Results: compared with the control group, the expression of miR-124 after operation was studied by flow cytometry. The cerebral cortical blood flow experienced the dynamic change of decreasing first and then rising. However, miR-124 showed a dynamic process of decreasing first and then rising, reaching the lowest level on the 7th day after operation. Overexpression of miR-124 can block SH-SY5Y of neural cell line in G1 phase. Conclusion: miR-124 may be involved in the development of cerebral ischemia after cerebral perfusion insufficiency in rats, which provides a new therapeutic strategy for the treatment of cerebral ischemia.
【作者单位】：重庆医科大学基础医学院病理学教研室重庆医科大学神经科学中心重庆市神经生物学重点实验室;
【分类号】：R743.3

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