β-石竹烯通过抗氧化作用减轻大鼠局灶性脑缺血再灌注损伤
发布时间：2018-04-03 10:20
本文选题：β-石竹烯 切入点：脑缺血再灌注 出处：《重庆医科大学》2016年硕士论文
【摘要】：目的:探讨β-石竹烯(β-Caryophyllene,BCP)对脑缺血再灌注(ischemiareperfusion,I-R)损伤大鼠的保护作用及其机制。观察其对核因子相关因子2(2NF-E2-related factor,Nrf2)/血红素氧合酶1(Heme oxygenase-1,HO-1)通路的作用。方法:1.将78只SD大鼠随机分为六组:假手术(Sham)组;模型(I-R)组;β-石竹烯34mg·kg-11组;β-石竹烯102 mg·kg-1组;β-石竹烯306mg·kg-1组;尼莫地平组6mg·kg-1。采用线栓法建立大鼠大脑中动脉栓塞(Middle Cerebral Artery Occlusion,MCAO)模型,缺血1.5h,再灌注24h后,采用双盲法进行神经行为学评分;TTC染色法测定脑梗死体积;透射电镜(Transmission electron microscopy,TEM)和尼氏染色法(Nissl)观察海马神经元病理变化;原位末端凋亡法(TUNEL)检测海马神经元凋亡情况;2.将60只SD大鼠随机分为五组:假手术(Sham)组;模型组(I-R);β-石竹烯34 mg·kg-1组;β-石竹烯102 mg·kg-1组;β-石竹烯306 mg·kg-1组。测定MCAO大鼠海马中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、丙二醛(Malondialdehyde,MDA)、一氧化氮(Nitric oxide,NO)、脂质过氧化物(Lipid peroxidation,LPO)含量;Western法和定量PCR法检测假手术(Sham)组;模型组(I-R);β-石竹烯306 mg·kg-1组Nrf2及HO-1蛋白及基因的表达。结果:1.与Sham组相比,I-R组神经行为学评分升高,脑梗死面积明显增加。β-石竹烯(102mg·kg-1、306 mg·kg-1)与尼莫地平组可不同程度改善MCAO大鼠神经行为学障碍,减少脑梗死面积;同时,β-石竹烯(102mg·kg-1、306 mg·kg-1)与尼莫地平组可减轻海马神经元病理缺损及细胞凋亡情况;2.与Sham组相比,I-R组SOD、CAT活性明显降低,MDA、LPO、NO含量明显增多。与I-R组相比,β-石竹烯(102mg·kg-1、306 mg·kg-1)中,SOD、CAT活性明显增高,MDA、LPO、NO含量明显降低;β-石竹烯(306mg·kg-1)Nrf2、HO-1蛋白及基因的表达明显增多。结论:β-石竹烯对脑缺血再灌注大鼠具有神经保护作用,此作用可能与调控Nrf2/HO-1通路有关。
[Abstract]:Aim: to investigate the protective effect of 尾 -Caryophyllene (尾 -Caryophyllene BCP) on cerebral ischemia-reperfusion injury in rats and its mechanism.To investigate the effect of NRF _ 2 / 1(Heme oxygenase 1(Heme oxygenase-1 (HO-1) pathway on the nuclear factor-related factor 2(2NF-E2-related factor-nrf2 / heme oxygenase 1 (1(Heme oxygenase-1) pathway.Method 1: 1.Seventy-eight SD rats were randomly divided into six groups: sham-operated group; model I-Rgroup; 尾 -caryophylene 34mg kg-11 group; 尾 -caryophyllene 102mg kg-1 group; 尾 -caryophylene 306mg kg-1 group; Nimodipine group 6mg kg-1.The middle Cerebral Artery occlusion model of middle cerebral artery (MCAO) was established by thread occlusion. After 1.5 hours of ischemia and 24 hours of reperfusion, the volume of cerebral infarction was measured by the method of neurobehavioral score and TTC staining.The pathological changes of hippocampal neurons were observed by transmission electron microscopy (TEM) and transmission electron microscopy (TEM) and Nissl staining (Nissll), and the apoptosis of hippocampal neurons was detected by in situ terminal terminal apoptosis (Tunel).Sixty SD rats were randomly divided into five groups: sham group, model group, 尾 -caryophyllene 34 mg kg-1 group, 尾 -caryophyllene 102mg kg-1 group, 尾 -caryophyllene 306 mg kg-1 group.娴嬪畾MCAO澶ч紶娴烽┈涓秴姘у寲鐗╂鍖栭叾(superoxide dismutase,SOD),杩囨哀鍖栨阿閰，
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