转化生长因子β激活激酶1介导的细胞自噬在小鼠脑缺血-再灌注损伤中的作用

发布时间：2018-04-12 08:55

  本文选题：转化生长因子β激活激酶 + 自噬　； 参考：《临床麻醉学杂志》2017年02期

【摘要】：目的评价转化生长因子β激活激酶1(TGFβ-activated kinase-1,TAK1)介导的细胞自噬在小鼠脑缺血-再灌注(ischemia-reperfusion,IR)损伤中的作用。方法雄性昆明小鼠72只,8周龄,体重(25.0±3.5)g,采用随机数字表法分为六组:空白对照组(C组)、假手术组(S组)、IR组、TAK1短发夹RNA(shRNA)慢病毒组(T组)、阴性对照慢病毒组(Y组)和生理盐水组(NS组),每组12只。T组、Y组和NS组分别以1μl/min侧脑室注射TAK1shRNA慢病毒、阴性对照慢病毒和等量生理盐水10μl。2周后IR组、T组、Y组和NS组制备脑IR损伤模型,缺血2h后恢复灌注;S组分离颈总动脉但不夹闭,其余手术步骤同IR组。再灌注24h后进行神经功能缺陷评分(neurological severity scores,NSS);NSS评分完成后处死小鼠,取脑组织测定脑梗死体积;Western blot法检测小鼠脑组织TAK1、LC3Ⅱ/LC3Ⅰ、Beclin1和p62蛋白含量。结果 IR组、T组、Y组和NS组NSS评分和脑梗死体积百分比明显高于C组(P0.05);T组NSS评分和脑梗死体积百分比明显低于IR组(P0.05)。IR组、Y组和NS组脑组织TAK1蛋白含量明显高于C组(P0.05);T组脑组织TAK1蛋白含量明显低于IR组(P0.05)。IR组、T组、Y组和NS组LC3Ⅱ/LC3Ⅰ比值、Beclin1蛋白含量明显高于C组(P0.05),p62蛋白含量明显低于C组(P0.05);T组脑组织LC3Ⅱ/LC3Ⅰ比值、Beclin1蛋白含量明显低于,p62蛋白含量明显高于IR组(P0.05)。结论 TAK1介导的细胞自噬参与小鼠脑缺血-再灌注损伤机制。
[Abstract]:Objective to evaluate the role of transforming growth factor 尾 -activated kinase (1(TGF 尾 -activated kinase-1 tak 1) -mediated autophagy in cerebral ischemia-reperfusion reperfusion injury in mice.Methods 72 male Kunming mice were 8 weeks old.TAK1shRNA lentivirus was injected into the lateral ventricle of 1 渭 l/min in group A and group NS, respectively.After 10 渭 l.2 weeks of negative control lentivirus and the same amount of normal saline, brain IR injury model was established in group T and group NS. The common carotid artery was isolated but not clipped in group S after 2 h ischemia. The other surgical procedures were the same as those in group IR.After 24 hours of reperfusion, the mice were killed by neurological severity scoress-NSSSS-NSS score. The contents of TAK1LC3 鈪，

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