VE-cadherin与N-cadherin在胶质瘤中的表达及意义

发布时间：2018-04-12 12:33

本文选题：VE-cadherin + N-cadherin　；参考：《泸州医学院》2014年硕士论文

【摘要】：目的：研究VE-cadherin与N-cadherin在胶质瘤中的表达及其与胶质瘤相关特性的关系,探讨两者在胶质瘤发生发展中的作用,为胶质瘤分子治疗提供理论依据。方法：①选择泸州医学院附属医院神经外科2011年1月—2013年1月部分胶质瘤手术切除后病理科存放的优质胶质瘤病理标本65例,所有的患者术前身体健康,选择18例颅脑外伤后行内外减压术患者的正常脑组织作为对照。②所获得胶质瘤组织及正常脑组织先行HE染色,再加用免疫组织化学技术,按照世界卫生组织(WHO)2007年中枢神经系统肿瘤分型、分级标准对胶质瘤进行分类。③标本行免疫组织化学染色后检测正常脑组织及肿瘤组织中VE-cadherin、N-cadherin的表达情况。④分析两指标在正常脑组织及胶质瘤中的表达情况,将指标表达强度进行分类。⑤从四个方面分析两指标的表达程度：正常脑组织和胶质瘤组织中的表达情况；在正常脑组织及不同级别胶质瘤组织中的表达情况；不同病理类型中胶质瘤组织的表达情况；在合并有不同程度瘤周水肿的胶质瘤组织中表达情况。然后进行统计分析。结果：①VE-cadherin在胶质瘤组织和正常脑组织中表达之间比较差异有显著性(Z=-3.640；P=0.0000.005),VE-cadherin在胶质瘤组织中表达高于正常脑组织。②VE-cadherin在正常脑组织、Ⅰ级、Ⅱ级、Ⅲ级和Ⅳ胶质瘤级之间表达比较差异有显著性(x2=22.181, P=0.0000.05), VE-cadherin表达强度与胶质瘤级别呈正相关(rs=0.491,P=0.0000.05)。③不同病理类型的胶质瘤组织中,VE-cadherin表达差异无显著性(x2=11.289, P=0.1260.005)。④VE-cadherin在合并无水肿、轻度水肿、中度水肿和重度水肿胶质瘤中表达差异有显著性(x2=21.257, P=0.0000.008), VE-cadherin表达强度与胶质瘤瘤周水肿程度呈正相关(r,=0.504, P=0.0000.05)。(?) N-cadherin在正常脑组织与胶质瘤组织中的表达程度差异有显著性(Z=-4.320, P=0.0000.05), N-cadherin在胶质瘤组织中的表达高于正常脑组织。⑥N-cadherin在正常脑组织、Ⅰ级、Ⅱ级、Ⅲ工级和工Ⅳ胶质瘤级之间表达比较差异有显著性(x2=35.246, P=0.0000.05), N-cadherin表达强度与胶质瘤级别呈正相关(rs=0.614, P=0.0000.05)。⑦在不同组织病理类型的胶质瘤中,N-cadherin表达差异无显著性(x2=11.958,P=0.1020.05)⑧N-cadherin在合并无水肿、轻度水肿、中度水肿和重度水肿胶质瘤中表达强度差异有显著性(x2=17.440,P=0.0010.05),N-cadherin表达强度与胶质瘤瘤周水肿程度呈正相关(rs=0.454,P=0.0000.05)。⑨VE-cadherin与N-cadherin在胶质瘤组织中的表达呈正相关(rs=0.206,P=0180.05)。结论：②VE-cadherin、 N-cadherin蛋白在胶质瘤组织和正常脑组织中表达差异有显著性,两者在胶质瘤组织中的表达均高于正常脑组织,且随着胶质瘤分级的增高而增高。提示他们协同通过某机制参与胶质瘤发生发展,影响胶质瘤的恶性程度。②VE-cadherin、 N-cadherin在不同胶质瘤组织病理类型中的表达差异无显著性,提示两者与胶质瘤的组织病理类型无关,也可能是本实验样本量小或者是选择偏倚所致,需进一步研究。③对VE-cadherin、N-cadherin在合并不同程度瘤周水肿胶质瘤组织中的表达研究发现,瘤周水肿越严重,两者的表达越明显,提示VE-cadherin、N-cadherin参与胶质瘤瘤周水肿的发生发展,加重胶质瘤病情。④对VE-cadherin与N-cadherin在胶质瘤组织中的表达相关性研究发现,VE-cadherin与N-cadherin在胶质瘤组织中的表达呈正相关,两者可能在胶质瘤组织中协同促进胶质瘤血管生成,影响胶质瘤的发生发展。
[Abstract]:Objective: To investigate the relationship between the expression of VE-cadherin and N-cadherin in gliomas and its correlation with glioma characteristics, discuss their role in the development of glioma, and provide a theoretical basis for the treatment of glioma. Methods: select the Department of Neurosurgery of Affiliated Hospital of Luzhou Medical College from January 2011 to January 2013 was part of glioma after surgery the pathology department store quality glioma 65 cases were all patients with preoperative health, 18 cases of craniocerebral trauma underwent decompressive craniotomy patients of normal brain tissue as control. The acquired glioma tissue and normal brain tissue to HE staining, and immunohistochemistry, in accordance with the WHO (WHO) 2007 central nervous system tumor type, the glioma classification standard. The specimens with immunohistochemical staining after detection of normal brain tissue and tumor tissue VE-cadherin, N-cadherin expression. The expression analysis of two indexes in normal brain tissue and glioma, the expression intensity index is used for classification. 5. Expression level two indexes from four aspects: the expression of normal brain tissues and glioma tissues; the expression in normal brain tissue and different grade glioma; expression of gliomas of different pathological type; expression of glioma with different degree of peritumoral edema in combined situation. Then analyze the results. Results: VE-cadherin had significant difference between the expression in glioma tissues and normal brain tissues (Z=-3.640; P=0.0000.005). The expression of VE-cadherin in glioma tissues than in normal tissues. The VE-cadherin in normal brain tissue, grade I, grade II, there are significant differences between the expression of glioma grade III and IV A (x2=22.181, P=0.0000.05), the expression of VE-cadherin was positively correlated with the level of glioma (rs=0.491, P=0.0000.05). The glioma tissues with different pathological types, the expression of VE-cadherin had no significant difference (x2=11.289, P=0.1260.005). The VE-cadherin is not in edema, mild edema, there was significant difference in the expression of moderate and severe edema edema of gliomas (x2=21.257, P=0.0000.008), the expression of VE-cadherin was positively correlated with the degree of peritumoral edema of gliomas (R, =0.504, P=0.0000.05). (?) there was significant difference between the expression level of N-cadherin in normal brain tissues and glioma tissues (Z=-4.320, P=0.0000.05), the expression of N-cadherin in glioma tissues than that in normal brain tissues. The N-cadherin in normal brain tissue, grade I, II, III and expression level IV glioma grade had significant difference compared (x2=35.246, P=0.0000.05), N The expression of -cadherin was positively correlated with the level of glioma (rs=0.614, P=0.0000.05). In the different types of pathology of glioma, the expression of N-cadherin had no significant difference (x2=11.958, P=0.1020.05) and N-cadherin in patients with mild edema, edema, edema and severe edema in gliomas expressed significant intensity differences (x2=17.440. P=0.0010.05), the expression of N-cadherin was positively correlated with the degree of peritumoral edema of gliomas (rs=0.454, P=0.0000.05). The expression of VE-cadherin was positively correlated with N-cadherin in glioma tissues (rs=0.206, P=0180.05). Conclusion: the VE-cadherin, N-cadherin protein was significantly expressed in glioma tissues and normal brain tissues, the expression of the two in glioma tissues were higher than those in normal brain tissue, and increased with the glioma grade. Suggesting that they co through a mechanism involved in glioma The occurrence and development of tumor, the malignant degree of glioma. The effects of VE-cadherin, N-cadherin expression in different pathological types of glioma tissues had no significant independent pathological types and suggests that the two gliomas, may be the amount of samples is small or due to selection bias, need further study. The expression of VE-cadherin. Research on N-cadherin with different degree of peritumoral edema in gliomas, peritumoral edema is more serious, the more obvious expression, suggesting that VE-cadherin and N-cadherin participate in the peritumoral edema development, exacerbation of glioma illness. The relationship between the expression of VE-cadherin and N-cadherin in glioma tissues found the positive expression of VE-cadherin and N-cadherin in glioma tissues, both of which may be synergistic in glioma glioma angiogenesis, influence of glioma Development.

【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.41

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