过表达BCL-2对急性脑梗死大鼠神经功能的影响

发布时间：2018-04-14 09:05

本文选题：脑梗死 + Bcl-　；参考：《中风与神经疾病杂志》2017年03期

【摘要】：目的探讨采用慢病毒介导Bcl-2在急性脑梗死大鼠脑组织过表达对神经功能的影响。方法 45只大鼠随机分为假手术组、模型组和治疗组。假手术组和模型组分别于手术前10 d通过颅内动脉给予慢病毒空载体,治疗组给予慢病毒Bcl-2过表达载体。在采用线栓法制作大鼠大脑中动脉脑梗死模型72 h后,分析大鼠脑组织梗死范围、脑细胞凋亡指数以及神经功能的变化。结果慢病毒介导的Bcl-2表达质粒可在大鼠脑组织中表达,治疗组大鼠脑组织中Bcl-2的水平明显高于假手术组和模型组;与假手术组比较,模型组和治疗组脑组织神经细胞凋亡指数和脑组织梗死范围明显增加(P0.05),而治疗组神经细胞凋亡指数和脑组织梗死范围较模型组明显下降(P0.05);治疗组大鼠神经功能损伤评分较模型组明显下降(P0.05)。结论过表达Bcl-2可降低大鼠脑组织神经细胞凋亡水平,对神经具有一定保护作用,为临床急性脑梗死治疗提供了一定的参考依据。
[Abstract]:Objective to investigate the effect of lentivirus-mediated Bcl-2 overexpression in the brain of rats with acute cerebral infarction.Methods 45 rats were randomly divided into sham operation group, model group and treatment group.The sham operation group and the model group were given lentivirus empty vector through intracranial artery 10 days before operation, while the treatment group received lentivirus Bcl-2 overexpression vector.After 72 hours of middle cerebral artery infarction in rats, the changes of infarct size, apoptosis index and neural function were analyzed.Results the expression plasmid of Bcl-2 mediated by lentivirus could be expressed in the brain tissue of rats. The level of Bcl-2 in brain tissue of treatment group was significantly higher than that of sham operation group and model group, and was higher than that of sham operation group.The neuronal apoptosis index and infarct size of brain tissue in model group and treatment group were significantly increased (P 0.05), while the apoptosis index and infarct size of brain tissue in treatment group were significantly lower than those in model group (P 0.05), and the nerve function damage in treatment group was significantly lower than that in model group (P < 0.05).The injury score was significantly lower than that in the model group (P 0.05).Conclusion overexpression of Bcl-2 can decrease the level of neuronal apoptosis in brain tissue of rats, and has a certain protective effect on nerve cells, which provides a certain reference for the treatment of acute cerebral infarction.
【作者单位】：河南科技大学第一附属医院神经内科;河南科技大学第一附属医院第一药房;河南科技大学第一附属医院神经外科;
【基金】：国家自然科学基金(No.U1304809) 河南洛阳市青年医学科技创新联合专项资金项目(No.1503008A-15)
【分类号】：R743.33

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