神经病理性疼痛致抑郁大鼠海马促炎细胞因子含量的变化

发布时间：2018-04-16 06:04

本文选题：神经病理性疼痛 + 抑郁　；参考：《临床麻醉学杂志》2017年08期

【摘要】：目的观察神经病理性疼痛致抑郁大鼠海马促炎细胞因子含量的变化,并探讨其与抑郁严重程度的关系。方法成年雄性SD大鼠28只,随机分为两组:假手术组(Sham组)和保留性坐骨神经损伤组(SNI组),每组14只。于术前1d及术后7、14、21d测试机械痛阈值(MWT);于术前1~3d测试糖水偏好、强迫游泳实验基础值;术后21~23d进行糖水偏好实验、强迫游泳实验;行为学测试结束后取海马组织,采用ELISA法检测海马中IL-1β、IL-6、TNF-α的含量,并对其与糖水偏好的相关性进行分析。结果与Sham组比较,SNI组大鼠在术后7、14、21d MWT明显降低(P0.001),术后21d糖水偏好明显降低(P0.01),术后23d强迫游泳不动时间明显延长(P0.05);术后21d海马组织IL-6、TNF-α含量明显升高(P0.05或P0.01),而IL-1β含量差异无统计学意义;SNI组海马组织IL-1β(r2=0.60,P0.01)、IL-6(r2=0.55,P0.01)、TNF-α(r2=0.60,P0.01)含量与糖水偏好呈明显负相关。结论神经病理性疼痛致抑郁大鼠海马促炎细胞因子IL-6、TNF-α含量升高,且与抑郁严重程度呈负相关。
[Abstract]:Objective to observe the changes of pro-inflammatory cytokines in hippocampus of depressive rats induced by neuropathic pain and to explore their relationship with the severity of depression.Methods Twenty-eight adult male Sprague-Dawley rats were randomly divided into two groups: sham-operated group (Sham group) and preserved sciatic nerve injury group (SNI group, 14 rats in each group).The mechanical pain threshold (MWTT) was measured 1 day before operation and 7 days after operation, and the basic value of forced swimming was measured on day 1 and day 3 before operation. The experiment was conducted on 21 ~ 23 days after operation, and the hippocampal tissue was taken from the hippocampus after behavioral test.The content of IL-1 尾 -IL-6 TNF- 伪 in hippocampus was detected by ELISA method, and the correlation between TNF- 伪 and sugar water preference was analyzed.Results compared with the Sham group, the MWT decreased significantly on the 7th day after operation, the preference for sugar water decreased significantly on the 21st day after operation, the immobility time of forced swimming was significantly prolonged at 23 days after operation, and the content of IL-6 TNF- 伪 in hippocampal tissue increased significantly on the 21st day after operation (P0.05 or P0.01), while the content of IL-1 尾 was significantly increased.There was no significant difference in the content of IL-1 尾 -r2O0.60P0.01P0.01in hippocampal tissue of SNI group. There was a significant negative correlation between the content of TNF- 伪 -0.60P0.01and the preference for sugar water.Conclusion the level of IL-6 TNF- 伪 in hippocampus of depression rats induced by neuropathic pain is increased and negatively correlated with the severity of depression.
【作者单位】：徐州医科大学江苏省麻醉学重点实验室江苏省麻醉与镇痛应用技术重点实验室;东南大学附属中大医院麻醉科;南京总医院;
【基金】：国家自然科学基金(81571083)
【分类号】：R741

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