化学方法体外诱导大鼠骨髓间充质干细胞成神经样细胞分化

发布时间：2018-04-19 06:55

本文选题：骨髓间充质干细胞 + 神经细胞　；参考：《生物技术通讯》2017年06期

【摘要】：目的:研究化学方法体外诱导大鼠骨髓间充质干细胞向神经样细胞的可持续性分化。方法:体外培养大鼠骨髓间充质干细胞至P5代,流式细胞术检测细胞表面标志物,将细胞分为丁羟茴醚(BHA)诱导组、β巯基乙醇(BME)诱导组和对照组,分别进行化学诱导分化,通过荧光定量PCR、Western印迹和免疫细胞化学法分别检测巢蛋白、神经元特异性烯醇化酶(NSE)、胶质原纤维酸性蛋白(GFAP)等神经细胞标志分子的表达情况。结果:BHA诱导组高表达GFAP,而BME诱导组高表达巢蛋白,2组NSE表达均较弱;同时在诱导结束后继续培养的过程中,2组神经标志分子表达量持续降低。结论:2种方法都能诱导骨髓间充质干细胞向神经样细胞分化,但分化结局不同,BHA诱导分化后细胞主要表达星形胶质细胞表面标志GFAP,而BME诱导分化后细胞主要表达神经前体细胞表面标志巢蛋白;此外,通过化学方法诱导分化的效果是不可持续的,在诱导结束后继续培养的过程中神经标志分子表达逐渐下调。
[Abstract]:Aim: to study the sustainable differentiation of rat bone marrow mesenchymal stem cells into neural like cells induced by chemical method in vitro.Methods: rat bone marrow mesenchymal stem cells were cultured to P5 passage in vitro. The cell surface markers were detected by flow cytometry. The cells were divided into three groups: BHA-induced group, BME-induced group and control group. The cells were chemically induced and differentiated.The expression of nestin, neuron-specific enolase (NSEE) and glial fibrillary acidic protein (GFAP) were detected by Western blot and immunocytochemistry, respectively.Results the expression of GFAP was higher in BHA group than that in NSE group in BME group, and the expression of nerve marker molecules in group 2 decreased continuously during the further culture after the induction.Conclusion both methods can induce the differentiation of bone marrow mesenchymal stem cells into neural like cells.However, the cells with different differentiation outcomes mainly expressed the astrocyte surface marker GFAP, while the BME induced differentiation cells mainly expressed the neural precursor cell surface marker nestin.The effect of inducing differentiation by chemical method was not sustainable, and the expression of neural markers was gradually down-regulated during the process of further culture after induction.
【作者单位】：郑州大学第一附属医院神经内科;
【基金】：河南省卫生厅中青年科技创新人才项目
【分类号】：R329.2

【相似文献】