miR-638通过靶向调控Notch 4促进神经胶质瘤细胞的侵袭能力

发布时间：2018-04-19 14:01

本文选题：神经胶质瘤 + 微小RNA-　；参考：《安徽医科大学学报》2017年01期

【摘要】：目的研究微小RNA-638(miR-638)在恶性胶质瘤中的表达,探讨miR-638在胶质瘤细胞侵袭过程中的作用及可能机制。方法实时荧光定量PCR(qRT-PCR)分别检测正常神经组织、胶质瘤组织、正常胶质细胞株、胶质瘤细胞株中miR-638的表达水平;Transwell实验检测分别转染miR-638模拟物和miR-638抑制物后U251细胞侵袭水平的改变;生物信息学预测和验证miR-638对Notch 4的靶向调控关系;构建Notch 4真核表达重组质粒(p CMV-Notch 4 c DNA)过表达U251细胞中的Notch 4表达水平,并检测U251细胞侵袭水平的改变。结果 miR-638在胶质瘤组织和细胞株中表达上调,而Notch 4在胶质瘤细胞中表达下调,两者表达呈负相关性;过表达miR-638使U251细胞的侵袭细胞数上升(P0.01),而抑制miR-638表达后U251细胞的侵袭细胞数减少(P0.01);miR-638在U251细胞中能直接靶向抑制Notch 4的表达,提升Notch 4在U251细胞中的表达能使侵袭细胞数减少(P0.01)。结论 miR-638在胶质瘤中高表达,其可能通过靶向抑制Notch 4的表达促进胶质瘤细胞的侵袭能力。
[Abstract]:Objective to study the expression of minimal RNA-638 miR-638) in malignant gliomas and to explore the role of miR-638 in the invasion of glioma cells and its possible mechanism.Methods the expression level of miR-638 in normal nerve tissue, glioma tissue, normal glial cell line and glioma cell line was detected by real-time fluorescence quantitative PCRQ RT-PCR. The invasion level of U251 cells was detected by Transwell assay after transfection of miR-638 mimic and miR-638 inhibitor respectively.Bioinformatics was used to predict and verify the targeted regulation of miR-638 on Notch 4, and to construct the eukaryotic expression plasmid of Notch 4, the expression level of Notch 4 in U251 cells, and to detect the change of invasion level of U251 cells.Results the expression of miR-638 was up-regulated in glioma tissues and cell lines, while the expression of Notch _ 4 was down-regulated in glioma cells.Overexpression of miR-638 increased the number of invasive cells in U251 cells, but decreased the number of invasive cells in U251 cells after inhibiting the expression of miR-638. The overexpression of miR-638 could directly inhibit the expression of Notch 4 in U251 cells, and elevating the expression of Notch 4 in U251 cells could reduce the number of invasive cells (P0.01).Conclusion miR-638 is highly expressed in gliomas, which may promote the invasion ability of glioma cells by targeting the expression of Notch 4.
【作者单位】：安徽医科大学附属省立医院肿瘤放疗科;滁州城市职业学院外科教研室;滁州城市职业学院内科教研室;
【基金】：安徽省自然科学基金(编号:1508085SMH233)
【分类号】：R739.4

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