烟雾病患者血清及脑脊液中VEGF和HGF的变化

发布时间：2018-04-22 11:46

本文选题：烟雾病 + 血管内皮生长因子　；参考：《泰山医学院》2014年硕士论文

【摘要】：目的烟雾病是一种原因不明的慢性进行性闭塞性脑血管疾病，主要表现为颈内动脉远端和大脑前或中动脉近端狭窄或闭塞、伴脑底部异常血管网形成，其病因尚不明确。机体内血管生成受多种细胞因子调控，根据其不同调控作用，可分为两大类：促血管生成因子及血管生成抑制因子。促血管生成因子指的是具有刺激血管生长的蛋白质分子或化合物。通过比较促血管生成因子中的血管内皮生长因子(VEGF)和肝细胞生长因子(HGF)在烟雾病患者血清及脑脊液中同非烟雾病患者之间含量的差异，探索VEGF及HGF在烟雾病血管改变机制中的起到的作用。方法选取2013年1月至2013年12月在我院神经外科住院的烟雾病患者24例为实验组，所有患者均经DSA检查确诊为烟雾病。同期选取因外伤导致蛛网膜下腔出血患者20例为对照组，该组患者均行颅脑MRI及经颅多普勒超声TCD检查排除烟雾病可能。所有入组患者均行腰椎穿刺术留取2ml脑脊液标本，均在清晨空腹时抽取肘静脉血5ml于抗凝管内，离心后留取血清标本，采用ELISA方法检测脑脊液及血清中VEGF及HGF的水平。结果 1、在脑脊液组中，VEGF及HGF含量的表达在烟雾病组合对照组间有显著性差异（p＜0.05），且在烟雾病组中的表达明显高于对照组（p＜0.05）； 2、在血清组中，VEGF及HGF含量的表达在烟雾病组合对照组间有显著性差异（p＜0.05），且在烟雾病组中的表达明显高于对照组（p＜0.05）。结论烟雾病患者血清及脑脊液中VEGF及HGF含量均增高，，提示VEGF及HGF在促进新生血管形成及保护营养神经方面具有重要作用，参与了烟雾病的病理生理过程。
[Abstract]:Purpose Moyamoya disease is a chronic progressive occlusive cerebrovascular disease with unknown causes. The main manifestations of moyamoya disease are stenosis or occlusion of the distal end of the internal carotid artery and anterior or proximal cerebral artery, accompanied by abnormal vascular network at the base of the brain. The etiology of moyamoya disease is still unclear. Angiogenesis is regulated by many cytokines and can be divided into two categories: angiogenic factors and angiogenic inhibitory factors. An angiogenic factor is a protein or compound that stimulates the growth of blood vessels. By comparing the levels of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in serum and cerebrospinal fluid of moyamoya disease patients with moyamoya disease and those without moyamoya disease, the difference between VEGF and HGF in serum and cerebrospinal fluid of moyamoya disease patients was compared. To explore the role of VEGF and HGF in the pathogenesis of moyamoya disease. Method Twenty-four patients with moyamoya disease from January 2013 to December 2013 were selected as experimental group. All patients were diagnosed as moyamoya disease by DSA. Twenty patients with subarachnoid hemorrhage caused by trauma were selected as control group. All the patients were examined with craniocerebral MRI and transcranial Doppler TCD to eliminate the possibility of moyamoya disease. All the patients underwent lumbar puncture and 2ml cerebrospinal fluid (CSF) samples were collected. 5ml was collected from the elbow vein blood in the anticoagulant tube on an empty stomach in the morning. Serum samples were collected after centrifugation. The levels of VEGF and HGF in CSF and serum were detected by ELISA method. Result 1. The expression of HGF and HGF in CSF group was significantly higher than that in the control group (p < 0.05), and was significantly higher in the moyamoya disease group than in the control group (p < 0.05). 2. The expression of HGF and HGF in the serum group was significantly higher than that in the control group (p < 0.05), and the expression in the moyamoya disease group was significantly higher than that in the control group (p < 0.05). Conclusion The contents of VEGF and HGF in serum and cerebrospinal fluid of patients with moyamoya disease were increased, suggesting that VEGF and HGF play an important role in promoting neovascularization and protecting nutritional nerve, and participate in the pathophysiological process of moyamoya disease.
【学位授予单位】：泰山医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.3

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