金属基质蛋白酶-2基因多态性与病毒性脑膜炎的易感性关系

发布时间：2018-04-24 14:24

本文选题：金属基质蛋白酶- + 遗传多态性　；参考：《现代预防医学》2017年06期

【摘要】：目的探讨金属基质蛋白酶-2(MMP-2)基因多态性与病毒性脑膜炎易感性之间的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法,测定186例病毒性脑膜炎患者和202例健康者的MMP-2基因启动子区-735C/T、-1306C/T、-790T/G和-1575G/A位点的基因多态性,通过2组的基因频率的比较,对比分析。结果 735C/T位点基因型频率(CC、CT、TT)在病例组中分别为63.44%、31.72%、4.84%,在对照组分别为49.01%、42.57%、8.42%,病例组的C等位基因频率为79.30%、T等位基因频率为20.70%,对照组则为70.30%、29.70%,差异有统计学意义(P0.05)。而-1306C/T、-790T/G、-1575G/A位点2组的各个基因型、等位基因频率之间的比较差异均无统计学意义(P0.05)。结论 MMP-2基因启动子区-735C/T位点多态性可能与病毒性脑膜炎的易感性有关,C基因可能是易感基因,T基因是保护基因。而-1306C/T、-790T/G和-1575G/A位点多态性和病毒性脑膜炎的易感性之间没有明确关系。
[Abstract]:Objective to investigate the relationship between the polymorphisms of the metalloproteinase -2 (MMP-2) gene and the susceptibility to viral meningitis. Methods the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used to determine the -735C/T, -1306C/T, -790T/G and -1575G/A loci in the MMP-2 promoter region of 186 patients with viral meningitis and 202 healthy persons. The genetic polymorphism of the 735C/T locus (CC, CT, TT) in the case group was 63.44%, 31.72%, 4.84%, respectively, 49.01%, 42.57%, 8.42% in the control group, and the frequency of C alleles of the case group was 79.30%, the T allele frequency was 20.70%, and the control group was 70.30%, 29.70%, and poor in the control group. The differences were statistically significant (P0.05). There was no significant difference in the frequencies of alleles between the 2 groups of -1306C/T, -790T/G and -1575G/A loci (P0.05). Conclusion the polymorphism of the -735C/T locus in the promoter region of the MMP-2 gene may be related to the susceptibility of viral meningitis, the C gene may be a susceptible gene, and the T gene is protected. There was no clear relationship between -1306C/T, -790T/G and -1575G/A polymorphism and susceptibility to viral meningitis.

【作者单位】：温州医科大学附属台州医院神经内科;
【基金】：浙江省台州市市级科研基金(1402ky01)
【分类号】：R512.3

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