阿托伐他汀片对脑缺血再灌注大鼠髓鞘碱性蛋白和神经胶质纤维酸性蛋白及神经元特异性烯醇化酶的影响

发布时间：2018-04-25 22:07

本文选题：脑缺血再灌注 + 阿托伐他汀　；参考：《中国临床药理学杂志》2017年04期

【摘要】：目的研究阿托伐他汀片预处理对脑缺血再灌注大鼠脑组织髓鞘碱性蛋白(MBP)、神经胶质纤维酸性蛋白(GFAP)和神经元特异性烯醇化酶(NSE)水平的影响。方法 30只雄性SD大鼠随机分为实验组、模型组、假手术组,每组10只。实验组术前5 d以阿托伐他汀混悬液5 mg·kg~(-1)·d~(-1)灌胃,每天1次;模型组、假手术组于术前5天以等量0.9%Na Cl溶液进行灌胃,每天1次。实验组、模型组建立右侧大脑中动脉闭塞缺血再灌注模型,假手术组仅分离右侧颈总动脉后清创缝合。各组分别于缺血再灌注24 h后取脑并用免疫组化方法检测梗死灶周围MBP、GFAP及NSE水平。结果假手术组脑组织NSE为0.18±0.02,GFAP为0.11±0.00,MBP为0.19±0.02;模型组NSE为0.11±0.03,GFAP为0.14±0.04,MBP为0.11±0.02,与假手术组相比,差异均有统计学意义(P0.05);实验组NSE为0.14±0.02,MBP为0.14±0.02,与模型组相比,差异有统计学意义(P0.05);实验组GFAP与模型组相比,差异无统计学意义(P0.05)。结论阿托伐他汀预处理有利于减轻脑缺血再灌注损伤,其作用可能与阿托他汀对大鼠神经元、少突胶质细胞的保护有关。
[Abstract]:Objective to study the effects of Atto vastatin tablet preconditioning on the levels of myelin basic protein (MBP), glial fibrillary acidic protein (glial fibrillary acidic protein) and neuron-specific enolase (NSE) in brain tissue of rats with cerebral ischemia reperfusion. Methods Thirty male Sprague-Dawley rats were randomly divided into experimental group, model group and sham operation group with 10 rats in each group. The rats in the experimental group were intragastrically perfused with Atto vastatin suspension (5 mg / kg / d) once a day on the 5th day before operation, while in the model group, the sham operation group was given the same amount of 0.9%Na Cl solution 5 days before operation, once a day. In the experimental group, the model group established the right middle cerebral artery occlusion ischemia reperfusion model, the sham operation group only separated the right common carotid artery after debridement and suture. The levels of GFAP and NSE were detected by immunohistochemical method after 24 hours of ischemia reperfusion in each group. Results the NSE of brain tissue in sham-operation group was 0.18 卤0.02GFAP 0.11 卤0.002MBP was 0.19 卤0.02, NSE in model group was 0.14 卤0.04mBP 0.11 卤0.02, compared with sham-operation group, the difference was significant (P0.05and NSE 0.14 卤0.02mBP, 0.14 卤0.02MBP, 0.14 卤0.02MBP, 0.14 卤0.02MBP, 0.14 卤0.02MBP, 0.14 卤0.02MBP, 0.14 卤0.02MBP, respectively). The difference was statistically significant (P 0.05), but there was no significant difference in GFAP between the experimental group and the model group (P 0.05). Conclusion Atto vastatin preconditioning can reduce cerebral ischemia-reperfusion injury, and its effect may be related to the protection of Atto statins on rat neurons and oligodendrocytes.
【作者单位】：广西壮族自治区人民医院神经内科;
【基金】：国家自然科学基金资助项目(81460192) 广西自然科学基金资助项目(2011GXNSFA018185)
【分类号】：R743

【相似文献】