原发性三叉神经痛患者外周血中部分促炎症因子水平的变化及意义

发布时间：2018-04-28 10:16

本文选题：三叉神经痛 + 白细胞介素-1β　；参考：《安徽医科大学》2015年硕士论文

【摘要】：目的三叉神经痛是口腔颌面部常见性及顽固性疾病之一,三叉神经痛又分为原发性三叉神经痛和继发性三叉神经痛。原发性三叉神经痛的发病原因一直不完全明确,存在多种假说及设想。本研究旨在探讨原发性三叉神经痛患者外周血中促炎症因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平的变化及意义。方法2013年1月~2014年9月就诊于安徽省口腔医院口腔外科及安徽合肥新民医院的原发性三叉神经痛患者30例作为三叉神经痛组,所选患者均符合第七版《口腔颌面外科学》三叉神经痛诊断标准,其中男13例,女17例;全身某些部位已明确诊断为恶性肿瘤者17例作为恶性肿瘤对照组,其中男8例,女9例;牙周和根尖周有明显炎症者17例作为普通炎症对照组,其中男7例,女10例;20例正常患者作为健康对照组,其中男9例,女11例;对照组患者均为就诊于安徽省口腔医院和安徽省立医院的患者。采集各组患者清晨空腹静脉血5 ml低温离心(4?C、3 500 r/min、10 min),分离血清后置于-70?C冰箱保存备用,采用双抗体夹心酶联免疫吸附试验(ELISA)检测30例三叉神经痛患者、17例恶性肿瘤患者、17例普通炎症患者以及20例健康患者清晨空腹静脉血血清中促炎症因子IL-1β、IL-6和TNF-α的表达水平。按照ELISA试剂盒中显示的操作步骤进行,然后绘制标准曲线,根据样品光密度值在标准曲线图上查出相应各促炎症因子水平,最后进行统计学处理。结果三叉神经痛患者外周静脉血血清中促炎症因子IL-1β、IL-6和TNF-α的表达水平均高于健康对照组,差异有统计学意义(p0.05);而与恶性肿瘤组及普通炎症组差异无统计学意义。结论三叉神经痛患者外周静脉血血清中促炎症因子IL-1β、IL-6和TNF-α的表达水平明显升高,提示炎症因子与三叉神经痛发病有一定关联。
[Abstract]:Objective trigeminal neuralgia is one of the common and obstinate diseases in oral and maxillofacial region. Trigeminal neuralgia is divided into primary trigeminal neuralgia and secondary trigeminal neuralgia. The etiology of primary trigeminal neuralgia is not completely clear, and there are many hypotheses and assumptions. The purpose of this study was to investigate the changes and significance of the levels of interleukin-1 尾 (IL-1 尾), interleukin-6 (IL-6) and tumor necrosis factor- 伪 (TNF- 伪) in peripheral blood of patients with primary trigeminal neuralgia. Methods from January 2013 to September 2014, 30 patients with primary trigeminal neuralgia were treated as trigeminal neuralgia group. All the selected patients met the diagnostic criteria of trigeminal neuralgia in the seventh edition of Oral and Maxillofacial surgery, including 13 males and 17 females. 17 patients with malignant tumors were definitely diagnosed as malignant tumors in some parts of the body, including 8 males. There were 17 cases of periodontal and periapical inflammation as normal control group, including 7 males and 10 females as normal control group, including 9 males and 11 females. All the patients in the control group were treated in Anhui Stomatological Hospital and Anhui Provincial Hospital. Fasting venous blood was collected from patients in each group in the morning. The blood samples were collected and stored in -70 C refrigerator for 10 mins. The expression of proinflammatory factor IL-1 尾 6 and TNF- 伪 in serum of 30 patients with trigeminal neuralgia and 17 patients with malignant tumor was detected by double antibody sandwich enzyme linked immunosorbent assay (Elisa). According to the operation steps shown in the ELISA kit, the standard curve was drawn, and the corresponding levels of pro-inflammatory factors were found out on the standard curve according to the optical density of the sample. Finally, statistical analysis was carried out. Results the levels of proinflammatory factor IL-1 尾 -IL-6 and TNF- 伪 in peripheral blood of patients with trigeminal neuralgia were significantly higher than those in healthy controls (P 0.05), but there was no significant difference between the patients with trigeminal neuralgia and the patients with malignant tumor and normal inflammation. Conclusion the expression of proinflammatory factor IL-1 尾-6 and TNF- 伪 in peripheral blood of patients with trigeminal neuralgia was significantly increased, suggesting that inflammatory factors may be associated with the pathogenesis of trigeminal neuralgia.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R745.11

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