miR-101对U87、U251胶质瘤细胞系功能影响的初步研究

发布时间：2018-04-29 22:20

本文选题：胶质瘤 + miR-101　；参考：《皖南医学院》2014年硕士论文

【摘要】：目的探讨miR-101对U87,U251胶质瘤细胞系功能影响的。为进一质瘤细胞系影响的机制奠定理论基础。方法通过合成miR-101转染U87,U251胶质瘤细胞系,观察转染后U87、U251在不同时间点中这些细胞系细胞增殖、周期、凋亡和侵袭能力的变化,并行统计学分析。采用10%的新生小牛血清培养U87、U251细胞,取对数生长期进行细胞实验,实验分为空白对照组,阴性对照组,阳性干预组；采用化学合成miR-101,通过瞬转法分别转染U87、U251细胞；采用MTT实验分析在不同时间点中这些细胞增殖情况的变化；采用流式细胞仪检测在不同时间点中这些细胞周期、凋亡情况的变化；采用Transwell实验和细胞划痕实验检测细胞体外侵袭情况的变化,分析其对胶质瘤细胞系功能影响。数据均以均数±标准差(x±s)表示,采用spss13.0统计软件进行统计学分析,以α=0.05为检验标准,各组数据间采用方差分析,P0.05表示差异有统计学意义。结果在U87和U251细胞中上调miR-101后,抑制细胞增殖,诱导胶质瘤细胞阻滞在G0／G1期,促使细胞凋亡增加明显和降低细胞侵袭与迁移能力。统计学分析miR-101对U87和U251胶质瘤细胞的抑制效果无统计学差异。结论miR-101是胶质瘤中的抑癌基因,过表达miR-101对人胶质瘤细胞系细胞功能可能有抑制作用；miR-101在体外过表达抑制胶质瘤细胞增殖、可诱导细胞阻滞于G0／G1期、促使细胞凋亡明显增加和降低细胞侵袭及迁移能力。
[Abstract]:Objective to investigate the effect of miR-101 on the function of U87 U251 glioma cell line. To lay a theoretical foundation for the mechanism of the effect of the tumor cell line. Methods U87 U251 glioma cells were transfected with miR-101. The changes of cell proliferation, cell cycle, apoptosis and invasion were observed at different time points after transfection. U87 U251 cells were cultured with 10% newborn calf serum. The cells were divided into blank control group, negative control group and positive intervention group. MTT assay was used to analyze the proliferation of these cells at different time points, and flow cytometry was used to detect the changes of cell cycle and apoptosis at different time points. Transwell assay and scratch assay were used to detect the changes of cell invasion in vitro and to analyze its effect on the function of glioma cell line. The data were all expressed as mean 卤standard deviation (x 卤s). The statistical analysis was carried out by spss13.0 statistical software, and the standard of 伪 -0. 05 was used as the test standard. There was significant difference between each group by means of ANOVA (P0.05). Results after upregulation of miR-101 in U87 and U251 cells, cell proliferation was inhibited, glioma cells were blocked in G0/G1 phase, apoptosis increased significantly and the ability of cell invasion and migration was decreased. There was no statistical difference in the inhibitory effect of miR-101 on U87 and U251 glioma cells. Conclusion miR-101 is a tumor suppressor gene in glioma. Overexpression of miR-101 may inhibit the function of human glioma cell line in vitro and inhibit the proliferation of glioma cell line. It can induce cell arrest in G0/G1 phase. Promote apoptosis to increase and reduce the ability of cell invasion and migration.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.41

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