胶原蛋白-明胶复合支架材料修复周围神经缺损

发布时间：2018-04-30 20:56

本文选题：胶原 + 明胶　；参考：《中国组织工程研究》2017年02期

【摘要】：背景:文献报道显示,胶原蛋白-明胶复合支架可促进周围神经缺损的早期恢复,但该结论尚未得到进一步证实。目的:观察胶原蛋白-明胶复合支架的细胞相容性及修复周围神经缺损的效果。方法:将胶原蛋白-明胶复合支架与SD大鼠骨髓间充质干细胞共培养5 d,观察两者复合形态。切除20只SD大鼠左侧30 mm的腓总神经,建立周围神经缺损模型。随机分组,实验组神经缺损处植入复合骨髓间充质干细胞的胶原蛋白-明胶复合支架,对照组植入自体神经,植入后16周,进行桥接体中段形态观察与电生理检测。结果与结论:1细胞与支架复合形态:骨髓间充质干细胞在支架上生长良好,与支架融合良好;2桥接体中段形态观察:两组有髓神经纤维密度比较差异无显著性意义(P0.05),实验组有髓神经纤维直径、髓鞘厚度及神经组织百分比显著低于对照组(P0.05);3电生理检测:两组运动神经传导速度、运动神经潜伏期、感觉神经传导速度、感觉神经波幅比较差异无显著性意义(P0.05),实验组运动神经波幅、感觉神经潜伏期显著低于对照组(P0.05);4结果表明:胶原蛋白-明胶复合支架具有良好的细胞相容性,可用于修复周围为神经缺损。
[Abstract]:Background: it has been reported that collagen-gelatin composite scaffolds can promote the early recovery of peripheral nerve defects, but this conclusion has not been further confirmed. Objective: to observe the cytocompatibility of collagen-gelatin composite scaffold and the effect of repairing peripheral nerve defect. Methods: collagen-gelatin composite scaffold and bone marrow mesenchymal stem cells of SD rats were co-cultured for 5 days. The peripheral nerve defect model was established by removing 30 mm common peroneal nerve on the left side of 20 SD rats. The experimental group was randomly divided into two groups: the experimental group was implanted with collagen-gelatin composite scaffold of bone marrow mesenchymal stem cells at the nerve defect and the control group was implanted with autologous nerve. The middle segment of the graft was observed by morphological observation and electrophysiological examination 16 weeks after implantation. Results and conclusion: bone marrow mesenchymal stem cells grew well on the scaffold. Morphological observation of the middle segment of the bridge between the two groups: there was no significant difference in the density of myelinated nerve fibers between the two groups, and the diameter of myelinated nerve fibers in the experimental group was not significantly different from that in the experimental group. The thickness of myelin sheath and percentage of nerve tissue were significantly lower than that of control group (P 0.05): motor nerve conduction velocity, motor nerve latency, sensory nerve conduction velocity were measured in both groups. There was no significant difference in the amplitude of sensory nerve. The amplitude of motor nerve and the latency of sensory nerve in the experimental group were significantly lower than those in the control group (P 0.05). The results showed that the collagen-gelatin composite scaffold had good cytocompatibility. It can be used to repair peripheral nerve defects.
【作者单位】：南阳市中心医院;郑州大学第一附属医院;
【分类号】：R318

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