IL-1β致痫大鼠GFAP的表达及其与癫痫的关系

发布时间：2018-05-02 16:14

本文选题：癫痫 + 胶质细胞　；参考：《青岛大学》2014年硕士论文

【摘要】：目的化学刺激wistar大鼠,建立癫痫模型,观察大鼠癫痫发作的特点及组织形态学改变；并在不同时间点观察星形胶质细胞胶质原纤维酸性蛋白(GFAP)在海马中的表达、分布的动态变化过程,探讨GFAP在脑室内注入白细胞介素1β(interleukin-1β,IL-1β)致癫痫模型海马区的表达及意义。方法健康雄性Wistar大鼠60只,分为2组：实验组(侧脑室注射IL-1β),对照组(侧脑室注射等量的生理盐水)。实验组分为发作组和非发作组,实验发作组分别在癫痫发作后7d、14d、21d.30d给予取脑。利用免疫荧光和Western Blot检测海马区GFAP的表达情况及随时间变化的规律。结果1.成功建立化学刺激大鼠癫痫模型。2.实验发作组GFAP的表达量高于对照组及实验未发作组；实验组内,致痫后30天表达达到高峰。结论1.在IL-1β致痫模型中,IL-1β可能导致星形胶质细胞增生；2.胶质细胞增生可能是癫痫发作的病理学基础,导致了癫痫的发生发展。
[Abstract]:Objective to establish an epileptic model by chemical stimulation of wistar rats, observe the characteristics and histomorphologic changes of epileptic seizures in rats, and observe the expression of glial fibrillary acidic protein (GFAP) in hippocampus at different time points. To investigate the expression and significance of GFAP in hippocampal region of epileptic model induced by intraventricular injection of interleukin-1 尾 interleukin-1 尾 (IL-1 尾). Methods 60 healthy male Wistar rats were divided into two groups: experimental group (lateral ventricle injection of IL-1 尾) and control group (lateral ventricle injection of the same amount of normal saline). The experimental group was divided into attack group and non-seizure group. The expression of GFAP in hippocampus was detected by immunofluorescence and Western Blot. Result 1. Successful establishment of chemically stimulated rat epileptic model. 2. 2. The expression of GFAP in the experimental attack group was higher than that in the control group and the non-seizure group, and the expression reached the peak 30 days after epilepsy in the experimental group. Conclusion 1. In IL-1 尾 induced epilepsy model, IL-1 尾 may lead to astrocyte proliferation. Glial proliferation may be the pathological basis of epileptic seizures, leading to the occurrence and development of epilepsy.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R742.1

【参考文献】