脑卒中后抑郁大鼠额叶、海马、杏仁核神经元数量变化

发布时间：2018-05-03 18:40

本文选题：脑卒中后抑郁 + 额叶　；参考：《山东医药》2017年19期

【摘要】：目的观察脑卒中后抑郁(PSD)大鼠大脑情感障碍相关脑区额叶、海马、杏仁核神经元数量变化。方法将20只健康成年雌性SD大鼠随机分为抑郁组、卒中组、PSD组和对照组,每组5只。抑郁组采用孤养法加以慢性不可预见的中等强度应激刺激(CUMS)制作抑郁模型。卒中组用线栓法建立脑卒中模型。PSD组先用线栓法建立脑卒中模型,然后用孤养法加以CUMS制作PSD模型。对照组大鼠正常饲养。给予CUMS后第29天处死各组大鼠,取额叶、海马、杏仁核区脑组织行神经元特异性核蛋白(NeuN)免疫荧光组织染色,光镜下计数并比较各组大鼠额叶、海马、杏仁核中神经元(NeuN阳性细胞)的数量。结果 PSD组大鼠情感障碍相关脑区额叶、海马、杏仁核中神经元数量分别为(98.20±1.78)、(43.00±7.87)、(93.00±3.53)个/视野,均低于其他三组(P均0.05)。结论 PSD组大鼠情感障碍相关脑区额叶、海马和杏仁核神经元数量减少。
[Abstract]:Objective to observe the changes of neurons in frontal lobe, hippocampus and amygdala in affective disorder related brain regions of rats with poststroke depression (PSD). Methods Twenty healthy adult female SD rats were randomly divided into depression group, stroke group and control group with 5 rats in each group. Depression model was established in depression group with chronic unpredictable moderate stress stimulation (CUMS). The stroke model was established by thread embolization method in stroke group. The stroke model was established by thread embolization method in PSD group, and then PSD model was made by CUMS. Rats in the control group were fed normally. On the 29th day after CUMS, the rats in each group were killed. The brain tissues of frontal lobe, hippocampus and amygdaloid area were stained with neuron-specific nucleoprotein Neun. The frontal lobe and hippocampus of each group were counted and compared under light microscope. The number of Neun positive neurons in the amygdala. Results the number of neurons in the frontal lobe, hippocampus and amygdala in the PSD group was 98.20 卤1.78 and 93.00 卤3.53 respectively, which was lower than that in the other three groups (P < 0.05). Conclusion the number of neurons in frontal lobe, hippocampus and amygdala decreased in PSD group.
【作者单位】：大理大学附属医院;大理大学临床医学院;
【基金】：国家自然科学基金资助项目(81360208)
【分类号】：R743.3;R749.4

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